Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠谷胱甘肽过氧化物酶，线粒体(GPX4)酶联免疫试剂盒（CSB-EL009869MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GPX4含量。GPX4是铁死亡过程中的关键调节因子，通过将GSH转化为GSSH来限制脂质过氧化，保护细胞免受铁死亡。其表达受NRF2调控，在多种肿瘤治疗中具有潜在应用价值。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域中对GPX4蛋白表达的动态监测，如铁死亡机制研究、抗氧化药物筛选、疾病模型构建及相关信号通路探索。为细胞生物学、基础医学研究提供灵敏稳定的分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

GPX4
Uniprot No.：

Q91XR9
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPX4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-95
1:2	Average %	100
	Range %	97-104
1:4	Average %	94
	Range %	85-97
1:8	Average %	98
	Range %	91-103

回收率：

The recovery of mouse GPX4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	92	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.