Mouse Osteocrin(OSTN) ELISA kit

Mouse Osteocrin(OSTN) ELISA kit

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中文名称：

小鼠骨织素(OSTN)酶联免疫试剂盒
货号：

CSB-EL017269MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠骨织素(OSTN)酶联免疫检测试剂盒（货号：CSB-EL017269MO），采用双抗体夹心法定量检测样本中OSTN的浓度。OSTN是一种由骨骼肌和骨细胞分泌的活性肽类激素，参与调节能量代谢平衡及骨重塑过程，在运动适应性反应和生物钟调控中发挥重要作用。该试剂盒可检测血清、血浆、细胞培养上清及组织匀浆等多种样本类型，检测灵敏度高，线性范围为15.6-1000 pg/mL，适用于能量代谢研究、骨发育调控机制探索、运动生理学实验等科研领域。实验采用预包被特异性抗体的酶标板，通过与样本中OSTN结合形成复合物，经显色反应实现精准定量，可广泛应用于肥胖模型、骨质疏松症研究或生物节律相关实验中的OSTN动态监测，为体外培养细胞或动物模型中的OSTN表达分析提供可靠工具。
别名：

Ostn ELISA Kit; Osteocrin ELISA Kit; Musclin) [Cleaved into: Processed Osteocrin] ELISA Kit
缩写：

OSTN
Uniprot No.：

P61364
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Stem Cells
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	87-97
1:10	Average %	95
	Range %	88-99
1:20	Average %	93
	Range %	87-100
1:40	Average %	95
	Range %	87-104

回收率：

The recovery of mouse OSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	85-99
EDTA plasma (n=4)	97	89-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.579	2.605	2.592	2.502
500	1.447	1.494	1.471	1.381
250	0.791	0.803	0.797	0.707
125	0.430	0.427	0.429	0.339
62.5	0.219	0.225	0.222	0.132
31.2	0.144	0.149	0.147	0.057
15.6	0.107	0.112	0.110	0.020
0	0.088	0.091	0.090

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Exercise-induced Musclin determines the fate of fibro-adipogenic progenitors to control muscle homeostasis J Lyu,Cell stem cell,2024

功能：

Hormone that acts as a ligand for natriuretic peptide receptor NPR3/NPR-C and promotes bone growth and physical endurance in muscle. Acts as a regulator of osteoblast differentiation and bone growth by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production. Required to enhance physical endurance: induced following physical exercise in muscle and promotes cGMP production, probably by interacting with NPR3/NPR-C. May act as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle.
基因功能参考文献：
1. The mechanism underlying the effect of OSTN on skeletal growth relevant to C-type natriuretic peptide /Natriuretic peptide receptor B signaling. PMID: 28990933
2. Data show that musclin (Ostn) production is stimulated by Ca2+-dependent proto-oncogene protein c-Akt (Akt) phosphorylation. PMID: 26668395
3. findings suggest that palmitate increases musclin gene expression via the activation of the PERK signaling pathway in C2C12 myotubes PMID: 26449458
4. osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator PMID: 14523025
5. Musclin expression level is tightly regulated by nutritional changes and its physiological role could be linked to glucose metabolism PMID: 15044443
6. Foxo1 downregulates musclin mRNA expression both in vitro and in vivo, which should explain insulin-mediated upregulation of this gene in muscle cells. PMID: 17950246
7. Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249
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亚细胞定位：

Secreted.
蛋白家族：

Osteocrin family
组织特异性：

Expressed in skeletal muscle and to a much lesser extent in bone, brown adipose tissue, spleen and testis. Not expressed in neurons.
数据库链接：

KEGG: mmu:239790

UniGene: Mm.390880

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.