Mouse Osteoclast-associated immunoglobulin-like receptor(OSCAR) ELISA kit

Mouse Osteoclast-associated immunoglobulin-like receptor(OSCAR) ELISA kit

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中文名称：

小鼠破骨细胞相关免疫球蛋白样受体(OSCAR)酶联免疫试剂盒
货号：

CSB-EL017255MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠破骨细胞相关免疫球蛋白样受体(OSCAR)酶联免疫试剂盒（CSB-EL017255MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OSCAR含量。小鼠破骨细胞相关免疫球蛋白样受体(OSCAR)在破骨细胞形成和免疫调节中起关键作用，能促进破骨细胞形成及存活，并参与免疫细胞的激活和成熟，是骨重建和免疫治疗的重要靶点。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，支持科研人员开展骨代谢调控机制、骨质疏松模型评估、骨免疫相关药物开发等基础研究，为体外实验提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Oscar ELISA Kit; Osteoclast-associated immunoglobulin-like receptor ELISA Kit; Osteoclast-associated receptor ELISA Kit; mOSCAR ELISA Kit; Ocl178 ELISA Kit
缩写：

OSCAR
Uniprot No.：

Q8VBT3
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

37.5 pg/mL-2400 pg/mL
灵敏度：

9.375 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSCAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	89
Range %	82-93
1:10	Average %	105
Range %	97-108
1:20	Average %	93
Range %	88-97
1:40	Average %	95
Range %	91-98

回收率：

The recovery of mouse OSCAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	91	85-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	2.885	2.877	2.881	2.761
1200	2.068	2.089	2.079	1.959
600	1.152	1.123	1.138	1.018
300	0.689	0.687	0.688	0.568
150	0.398	0.391	0.395	0.275
75	0.301	0.289	0.295	0.175
37.5	0.210	0.222	0.216	0.096
0	0.119	0.121	0.120

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

A novel autoantibody signatures for enhanced clinical diagnosis of pancreatic ductal adenocarcinoma T Li,Cancer cell international,2023
Caffeic Acid Phenethyl Ester Abrogates Bone Resorption in a Murine Calvarial Model of Polyethylene Particle-Induced Osteolysis Zawawi MS et al,Calcif Tissue Int,2015

功能：

Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation.
基因功能参考文献：
1. OSCAR expression in monocytic cells is regulated by proatherogenic stimuli further pointing towards a role in vascular inflammation or plaque vulnerability during atherosclerosis PMID: 23817038
2. Data suggest that OSCAR is a collagen receptor that binds to specific collagen motifs and costimulates osteoclastogenesis in DAP12-deficient humans and mice. PMID: 21841309
3. PU.1 and MITF transcription factors synergistically activate OSCAR gene expression, which is further enhanced by the TRANCE-induced MKK6/p38 signaling cascade. PMID: 12695521
4. the FcRgamma-mediated signal transduction by OSCAR is involved in osteoclast differentiation PMID: 15184345
5. analysis of a positive feedback circuit of TRANCE-induced activation of NFATc1, involving NFATc1-mediated OSCAR expression and its subsequent activation of NFATc1, necessary for efficient differentiation of osteoclasts PMID: 16109714
6. Results indicate that USFs 1 and 2 serve as modulators in the induction of OSCAR and RANKL-mediated osteoclastogenesis. PMID: 18762192
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亚细胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Leukocyte receptor complex/polymeric immunogobulin receptor (PIR/LRC) family
组织特异性：

Specifically expressed in preosteoclasts or mature osteoclasts.
数据库链接：

KEGG: mmu:232790

STRING: 10090.ENSMUSP00000041306

UniGene: Mm.358882

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.