Mouse Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit

Mouse Ornithine carbamoyltransferase, mitochondrial(OTC) ELISA kit

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

小鼠鸟氨酸氨甲酰基转移酶，线粒体(OTC)酶联免疫试剂盒
货号：

CSB-EL017270MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

小鼠鸟氨酸氨甲酰基转移酶，线粒体(OTC)酶联免疫试剂盒（CSB-EL017270MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OTC含量。试剂盒检测范围为3.12 mU/mL-200 mU/mL，适用于肝病模型研究、尿素循环代谢机制探索、基因工程动物模型验证等科研场景，可满足科研实验中定量分析OTC酶动态变化的需求，为代谢性疾病机制研究、药物肝毒性评估等提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Otc ELISA Kit; Ornithine carbamoyltransferase ELISA Kit; mitochondrial ELISA Kit; EC 2.1.3.3 ELISA Kit; Ornithine transcarbamylase ELISA Kit; OTCase ELISA Kit
缩写：

OTC
Uniprot No.：

P11725
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 mU/mL-200 mU/mL
灵敏度：

0.78 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OTC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	91
	Range %	86-97
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-100

回收率：

The recovery of mouse OTC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-109
EDTA plasma (n=4)	96	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
200	2.348	2.272	2.310	2.196
100	1.826	1.732	1.779	1.665
50	1.176	1.239	1.208	1.094
25	0.708	0.743	0.726	0.612
12.5	0.409	0.431	0.420	0.306
6.25	0.257	0.271	0.264	0.150
3.12	0.199	0.204	0.202	0.088
0	0.115	0.112	0.114

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.
基因功能参考文献：
1. Gene correction in adult OTC-deficient mice was lower and accompanied by larger deletions that ablated residual expression from the endogenous OTC gene, leading to diminished protein tolerance and lethal hyperammonemia on a chow diet PMID: 26829317
2. The corresponding OTC tissue enzyme activities were between 3-6% of normal control in mouse and human liver. The use of the cryptic splice sites was reproduced in minigenes carrying murine or human mutant sequences PMID: 25853564
3. Serum OCT seemed to reflect tumor necrosis factor-alpha-mediated hepatic damage in diabetic obese mice and could be useful in the application for non-alcoholic fatty liver disease with features of metabolic syndrome, such as obesity and diabetes. PMID: 19793175
4. in vivo regulation by HNF4alpha PMID: 11994307
5. Results highlight the importance of the interaction between the OTC spf-ash mutation (ornithine transcarbamylase deficiency) and genetic backgrounds on metabolic phenotypes (ureagenesis, arginine metabolism, and nitric oxide production). PMID: 17925451
6. Phenotypic correction of OTC deficiency using low-dose helper-dependent adenoviral vectors is reported. PMID: 18563850
7. Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals. PMID: 19318352
显示更多

收起更多
相关疾病：

Defects in Otc are the cause of the Sparse fur (spf) phenotype. Spf mouse have an OTCase with an overall decrease in activity, and altered substrate affinity.
亚细胞定位：

Mitochondrion matrix.
蛋白家族：

ATCase/OTCase family
数据库链接：

KEGG: mmu:18416

STRING: 10090.ENSMUSP00000056152

UniGene: Mm.2611

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.