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Mouse Noradrenaline,NA ELISA Kit

  • 中文名称:
    小鼠去甲肾上腺素(NA)酶联免疫试剂盒
  • 货号:
    CSB-E07870m
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

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  • 产品描述:
    CUSABIO小鼠去甲肾上腺素(NA)酶联免疫检测试剂盒(货号:CSB-E07870m),采用竞争法原理实现定量分析,适用于科研实验中血清、血浆样本的精确检测。去甲肾上腺素作为重要的儿茶酚胺类神经递质和激素,在交感神经系统激活、心血管调节及应激反应中发挥关键作用,其水平变化常作为神经系统功能、心血管疾病模型及药物干预研究的生物标志物。本试剂盒检测范围为7.5-300 pg/mL,最低检测灵敏度达7.5 pg/mL,通过预包被特异性抗体与样本/标准品中的NA竞争结合酶标抗原,经显色反应后测定吸光度值,结合标准曲线计算目标物浓度。该试剂盒适用于神经生物学研究、应激反应机制探索、心血管药理学实验中对NA分泌水平的动态监测,也可用于评估抗高血压药物或神经调节剂对NA代谢通路的影响。产品提供完整的检测组分和优化实验方案,具备高特异性与批次稳定性,标准曲线线性优异(R²>0.99),满足微量样本的高效检测需求。
  • 别名:
    N/A
  • 缩写:
    NA
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma
  • 检测范围:
    7.5 pg/mL-300 pg/mL
  • 灵敏度:
    7.5 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse noradrenaline in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 98-104  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 86  
    Range % 83-89  
    1:8 Average % 91  
    Range % 88-94  
  • 回收率:
    The recovery of mouse noradrenaline spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 82-89  
    EDTA plasma (n=4) 97 94-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    300 0.371 0.396 0.384    
    150 0.548 0.578 0.563    
    69 0.775 0.798 0.787    
    22.5 1.126 1.168 1.147    
    7.5 1.672 1.587 1.630    
    0 2.201 2.209 2.205    
  • 数据处理:
  • 货期:
    3-5 working days

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