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中文名称:小鼠去甲肾上腺素(NA)酶联免疫试剂盒
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货号:CSB-E07870m
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规格:96T/48T
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价格:¥3800/¥2500
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其他:
产品详情
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产品描述:CUSABIO小鼠去甲肾上腺素(NA)酶联免疫检测试剂盒(货号:CSB-E07870m),采用竞争法原理实现定量分析,适用于科研实验中血清、血浆样本的精确检测。去甲肾上腺素作为重要的儿茶酚胺类神经递质和激素,在交感神经系统激活、心血管调节及应激反应中发挥关键作用,其水平变化常作为神经系统功能、心血管疾病模型及药物干预研究的生物标志物。本试剂盒检测范围为7.5-300 pg/mL,最低检测灵敏度达7.5 pg/mL,通过预包被特异性抗体与样本/标准品中的NA竞争结合酶标抗原,经显色反应后测定吸光度值,结合标准曲线计算目标物浓度。该试剂盒适用于神经生物学研究、应激反应机制探索、心血管药理学实验中对NA分泌水平的动态监测,也可用于评估抗高血压药物或神经调节剂对NA代谢通路的影响。产品提供完整的检测组分和优化实验方案,具备高特异性与批次稳定性,标准曲线线性优异(R²>0.99),满足微量样本的高效检测需求。
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别名:N/A
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缩写:NA
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma
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检测范围:7.5 pg/mL-300 pg/mL
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灵敏度:7.5 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Neuroscience
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse noradrenaline in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 100 Range % 98-104 1:2 Average % 87 Range % 84-90 1:4 Average % 86 Range % 83-89 1:8 Average % 91 Range % 88-94 -
回收率:
The recovery of mouse noradrenaline spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 85 82-89 EDTA plasma (n=4) 97 94-100 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 300 0.371 0.396 0.384 150 0.548 0.578 0.563 69 0.775 0.798 0.787 22.5 1.126 1.168 1.147 7.5 1.672 1.587 1.630 0 2.201 2.209 2.205 -
数据处理:
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货期:3-5 working days
引用文献
- Chronic stress disturbed the metabolism of homocysteine in mouse hippocampus and prefrontal cortex C Xue, B Liu, Y Zhao, X Wang, ZW Sun, F Xie, LJ Qian,Neuroscience,2024
- UVB Induces Sympathetic Nervous System Activation and Norepinephrine Secretion to Regulate The Skin Color of Mice Through the β2-AR/AP-1 Pathway in Epidermal Keratinocytes Q Deng, X Liu, X Wen, H Huang, H Tang,Inflammation,2025
- CAR T cells secreting NGF-neutralizing scFv enhance efficacy in clear cell renal cell carcinoma by relieving immunosuppression through immunosympathectomy P Yang, X Chen, F Yu, L Wang, M Li,Journal for Immunotherapy of Cancer,2024
- Exercise-induced anxiety impairs local and systemic inflammatory response and glucose metabolism in C57BL/6J mice I Gálvez, C Navarro, S Torres-Piles,Brain, Behavior, & Immunity-Health,2024
- Sleep Deprivation Triggers the Excessive Activation of Ovarian Primordial Follicles via β2 Adrenergic Receptor Signaling L Weng, H Hong, Q Zhang, C Xiao, Q Zhang,Advanced Science,/
- Immunoneuroendocrine, Stress, Metabolic, and Behavioural Responses in High-Fat Diet-Induced Obesity M del Carmen Navarro,Nutrients,2024
- Effect of quercetin on the progression of breast cancer in mice with chronic stress by regulating the polarization of microglia T Luo,Journal of Functional Foods,2024
- Peripheral administration of lipidized NPAF and NPFF analogs does not influence central food intake regulation but induces anxiety-like behavior V Strnadová,Neuropeptides,2024
- Kaempferol Improves Breast Cancer-Related Depression through the COX-2/PGE2 Pathway Q Zhu,IMR Press,2023
- Combined SGLT1 and 2 Inhibition Os Associated With Improved Glucose Control and Multi-Organ Protection in the Type 1 Diabetic Akimba Mouse L Herat,SSRN,2023
- Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9 R Ramendra,iScience,2024
- SGLT1/2 inhibition improves glycemic control and multi-organ protection in type 1 diabetes. LY Herat,iScience,2023
- LncRNA Nron deficiency protects mice from diet-induced adiposity and hepatic steatosis B Liu,Metabolism,2023
- The Sympathetic Nervous System Regulates Sodium Glucose Co-Transporter 1 Expression in the Kidney J Matthews,Biomedicines,2023
- Hypothermia evoked by stimulation of medial preoptic nucleus protects the brain in a mouse model of ischaemia S Zhang,Nature communications,2022
- Local hyperthermia therapy induces browning of white fat and treats obesity Yu Li,Cell,2022
- Aminergic status in the brain of urokinase gene-deficient mice with malignant tumors growing in presence of chronic neurogenic pain YA Pogorelova,Journal of Clinical Oncology,2021
- Оказывает ли влияние изменение нейротрансмиттерного статуса мозга на рост перевивной меланомы? ОИ Кит,/,2021
- SGLT2 Inhibitor-Induced Sympathoexcitation in White Adipose Tissue: A Novel Mechanism for Beiging. JR Matthews,Biomedicines,2020
- Intestinal microbiota contributes to altered glucose metabolism in simulated microgravity mouse model Wang Y, et al,Faseb Journal,2019
- Intrauterine exposure to hyperglycemia retards the development of brown adipose tissue Yu DQ, et al,Faseb Journal,2019
- Acute tobacco smoke exposure exacerbates the inflammatory response to corneal wounds in mice via the sympathetic nervous system Xiao C, et al,Acta Crystallographica Section F-Structural Biology Communications,2019
- IL-25 induces beige fat to improve metabolic homeostasis via macrophage and innervation Lingyi Li, et al,BioRxiv,2018
- AMP-Activated Protein Kinase (AMPK) Regulates Energy Metabolism through Modulating Thermogenesis in Adipose Tissue Lingyan Wu.et al,ORIGINAL RESEARCH ARTICLE,2018
- p38α function in osteoblasts influences adipose tissue homeostasis. Rodr铆guez-Carballo E.et al,FASEB J.,2015
- NMDA Receptor Antagonist Attenuates Bleomycin-Induced Acute Lung Injury. Yang Li, et al,PLoS One,2015
- Curcumin promotes browning of white adipose tissue in a norepinephrine-dependent way Wang S. et al,Biochem Biophys Res Commun,2015
- Intestinal gluconeogenesis is crucial to maintain a physiological fasting glycemia in the absence of hepatic glucose production in mice Penhoat A et al,Metabolism,2013
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