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  4. Mouse Neutrophil defensin 4(DEFA4) ELISA kit

Mouse Neutrophil defensin 4(DEFA4) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠中性粒细胞防御素4(DEFA4)酶联免疫试剂盒
  • 货号：
    CSB-EL006656MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠中性粒细胞防御素4(DEFA4)酶联免疫试剂盒（CSB-EL006656MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DEFA4含量。DEFA4是一种靶点。它在生物体内可能具有特定生理功能。目前对于它的研究，主要聚焦于其在相关生理和病理过程中的作用机制，探索它与疾病发生发展的联系，为开发针对该靶点的治疗手段、药物等提供理论依据。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域中小鼠模型感染性疾病机制研究、炎症反应评估或免疫调控实验，例如通过检测不同病理状态下DEFA4的表达水平变化，探索其在宿主防御系统中的作用，亦可用于药物开发中免疫调节剂的效果验证。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Defa4 ELISA kit; Defcr4Alpha-defensin 4 ELISA kit; Defensin-related cryptdin-4 ELISA kit
  • 缩写：
    DEFA4
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse DEFA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 85
    Range % 81-89
    1:2 Average % 93
    Range % 90-96
    1:4 Average % 88
    Range % 84-92
    1:8 Average % 101
    Range % 97-105
  • 回收率：
    The recovery of mouse DEFA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 95-104
    EDTA plasma (n=4) 83 80-86
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.654 2.689 2.672 2.515
    10 2.059 2.001 2.030 1.873
    5 1.312 1.335 1.324 1.167
    2.5 0.852 0.868 0.860 0.703
    1.25 0.505 0.512 0.509 0.352
    0.625 0.373 0.360 0.367 0.210
    0.312 0.266 0.268 0.267 0.110
    0 0.158 0.156 0.157 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Defa4 Proteins

靶点详情

  • 功能：
    Probably contributes to the antimicrobial barrier function of the small bowel mucosa.
  • 基因功能参考文献：
    1. Depleting CRP4 is incompatible with a proper cardiac development and function. CRP4 is a novel antiremodeling factor regulated, at least to some extent, by cardiac cGMP. PMID: 28138039
    2. Backbone cyclization provides a means for engineeringalpha-defensins to improve their biological activity while providing excellent stability to serum degradation and highlighting the potential of peptide scaffolds for development of novel compounds. PMID: 22040603
    3. bactericidal activity predominantly affects noncommensal bacteria PMID: 21099205
    4. the prosegment of Crp4 inhibits Crp4 bactericidal activity by blocking peptide-mediated perturbation of target cell membranes PMID: 12574157
    5. Crp4 disulfide arrangement confers essential protection from degradation by this critical activating proteinase, allowing antibacterial action. PMID: 15297466
    6. function of the conserved salt bridge in Crp4 is not linked to bactericidal activity or proteolytic stability of the mature peptide. PMID: 16857681
    7. CRP4 mediates cGMP/PKG stimulation of SM-specific gene expression, and PKG plays an important role in regulating the phenotype of VSMCs PMID: 17878170
    8. Kinetics of Crp4 translocation coupled with peptide-induced vesicle leakage are reported. PMID: 17910476
    9. Beta-sheet-containing Crp4 induces membrane permeabilization more effectively than disulfide-null Crp4 by translocating and forming transient membrane defects. PMID: 18973303
    10. Lys-for-Arg replacements in cryptdin 4 attenuated bactericidal activity PMID: 19737896

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha-defensin family
  • 组织特异性：
    Paneth cells of the small bowel.
  • 数据库链接：

    KEGG: mmu:13238

    UniGene: Mm.426634

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |