Mouse Neuron-specific enolase,NSE ELISA Kit

Mouse Neuron-specific enolase,NSE ELISA Kit

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中文名称：

小鼠神经特异性烯醇化酶(NSE)酶联免疫试剂盒
货号：

CSB-E07962m
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠神经特异性烯醇化酶（NSE）酶联免疫试剂盒（货号：CSB-E07962m），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及细胞培养上清液样本中NSE蛋白的精准分析。NSE是糖酵解途径关键酶烯醇化酶的γ亚型异构体，主要分布于神经元和神经内分泌细胞中，其表达水平与神经损伤、神经内分泌肿瘤及神经退行性疾病密切相关，是神经生物学研究的重要分子标记物。本试剂盒检测范围为1.56-100 ng/mL，提供预包被捕获抗体的96孔板、生物素标记检测抗体及标准品等核心组分，通过显色反应实现目标蛋白浓度测定，实验流程包含标准品梯度稀释、样本孵育、洗涤及底物显色等标准化步骤，适用于神经损伤模型评估、神经细胞分化研究、神经相关疾病机制探索以及药物干预效果监测等科研领域，为神经科学基础研究提供稳定可靠的工具支持。
别名：

Eno2 ELISA Kit; Eno-2Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
缩写：

ENO2
Uniprot No.：

P17183
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	89-102
1:2	Average %	91
	Range %	87-95
1:4	Average %	94
	Range %	84-97
1:8	Average %	91
	Range %	85-94

回收率：

The recovery of mouse NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	92-108
EDTA plasma (n=4)	91	84-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.622	2.542	2.582	2.475
50	2.209	2.167	2.188	2.081
25	1.463	1.487	1.475	1.368
12.5	0.762	0.734	0.748	0.641
6.25	0.358	0.333	0.346	0.239
3.12	0.252	0.245	0.249	0.142
1.56	0.180	0.168	0.174	0.067
0	0.106	0.108	0.107

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Combined Effects of Particulate Matter Exposure and Exercise Training on Neuroplasticity-Related Growth Factors and Blood–Brain Barrier Integrity SY Cho, HT Roh,Atmosphere,2025
Dephosphorylation by calcineurin regulates translocation of dynamin-related protein 1 to mitochondria in hepatic ischemia reperfusion induced hippocampus injury in young mice Yu X, et al,Brain Research,2019

功能：

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
基因功能参考文献：
1. Study analyzed the regions that regulate the promoter strength and cellular specificity of the 1.8 kb rNSE promoter in the cerebellum in vivo. The rNSE promoter was divided into three regions (A, B, and C). Our results showed that region C was indispensable for the promoter activity in mouse cerebellum and served as an interneuron-predominant basal promoter. PMID: 28508170
2. Plaque-associated microglial cells express gamma-enolase and cathepsin X. PMID: 23621429
3. Treadmill exercise training decreased the expression of NSN in the striatum of chronic Parkinsonian mice, which can partially explain the beneficial neuroprotective role of exercise in patients with parkinson disease. PMID: 21725169
4. we induced differentiation of mouse stromal cells to neuron-like cells and observed the activation of tissue-specific promoter of neuron-specific enolase in transgenic mice with the human bcl-2 gene expressed only in neurons under the NSE promoter PMID: 14593212
亚细胞定位：

Cytoplasm. Cell membrane.
蛋白家族：

Enolase family
组织特异性：

Skeletal muscle (at protein level). The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodi
数据库链接：

KEGG: mmu:13807

STRING: 10090.ENSMUSP00000004378

UniGene: Mm.3913

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.