Mouse NACHT, LRR and PYD domains-containing protein 3(NLRP3) ELISA kit

As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF.

1. Results suggest that activation of mammalian target of rapamycin (mTOR)/reactive oxygen species (ROS)/NOD-like receptor protein 3 (NLRP3) signaling may contribute to the development of systemic lupus erythematosus (SLE). PMID: 30060081
2. Inflammasome stimuli can act as polarizing cues to initiate dynamic NLRP3 and MARK4 transport in macrophages. PMID: 28656979
3. CD36 might play an important role in podocyte apoptosis by activating the NLRP3 inflammasome in primary nephrotic syndrome. PMID: 30258045
4. Data indicate function of macrophage migration inhibitory factor (MIF) as a regulator of the NLR family pyrin domain containing 3 (NLRP3) inflammasome complex in macrophages. PMID: 29884801
5. In agreement with a possible neutrophil contribution to systemic inflammation in cryopyrin associated periodic syndrome, the levels of neutrophil secretory proteins were significantly elevated in the plasma from Nlrp3(A350V) mice. PMID: 29322034
6. NLRP3(R258W)-induced remodelling of the gut microbiota, induces local Tregs to maintain homeostasis and compensate for otherwise-detrimental intestinal inflammation. PMID: 29196621
7. Pyrin signaling is dispensable for Clostridium difficile infection (CDI) associated intestinal epithelial cells death and for in vivo pathogenesis; C. difficile enterotoxins induce activation of executioner caspases 3/7 via the intrinsic apoptosis pathway indicating that caspase-3/7-mediated intestinal epithelial cells apoptosis is critical for in vivo host defense during early stages of CDI. PMID: 30451870
8. The results of this study suggested that the upregulation of NLRP3 in DRG via STAT3-dependent histone acetylation is critically involved in bortezomib-induced mechanical allodynia. PMID: 29339053
9. sumoylation of NLRP3 restrains inflammasome activation, and identify SUMO proteases as potential drug targets for the treatment of inflammatory diseases. PMID: 30069026
10. Nephrocalcinosis-related chronic kidney disease involves NLRP3 but not necessarily via intrarenal IL-1 release but rather via other biological functions including TGFR signaling and macrophage polarization. PMID: 29241624
11. Study shows that the NLRP3 inflammasome was involved in the complex diseases of diabetic stroke. MCC950, the NLRP3 specific inhibitor, ameliorated diabetic mice with cerebral ischemia-reperfusion injury and improved the 28-day survival rate during the recovery stage of ischemic stroke. PMID: 29853850
12. NLRP3 inflammasome serves as a key player in the pathogenesis of IgA nephropathy (IgAN) partly through activation of T cells and mitochondrial ROS production and that a local, kidney-targeting suppression of NLRP3 be a therapeutic strategy for IgAN. PMID: 28117341
13. Study in mice models shows that perforin from antigen-specific cytotoxic T lymphocytes is required for Nlrp3 inflammasome activation in antigen-presenting cells. Furthermore, such activation of NLRP3 inflammasome contributes to the induction of antigen-specific antitumour immunity and pathogenesis of graft-versus-host diseases. PMID: 28537251
14. The results show that the NLRP3 inflammasome impairs host defense during lethal pneumonia caused by serotype 3 Streptococcus pneumoniae . PMID: 28971472
15. this study shows that soluble uric acid activates the NLRP3 inflammasome PMID: 28084303
16. These findings indicate that the NAD(+)/SIRT2 pathway is an important mediator through which silybin prevents NLRP3 inflammasome activation during NAFLD PMID: 28970254
17. NEK7 is a specific K(+) sensor and does not associate with NLRP3 under conditions stimulating exclusively Cl(-) efflux, but does after K(+) efflux, activating the complex driving inflammation. PMID: 30232264
18. miR155 expression levels were significantly increased in an in vivo model of psoriasis compared with normal tissues, as was the expression of NLR family pyrin domain containing 3 (NLRP3). PMID: 29767259
19. Anaplastic lymphoma kinase (ALK) is a novel regulator of NLRP3 inflammasome activation in macrophages. Mechanically, ALK-mediated NF-kappaB activation was required for the priming step of NLRP3 upregulation, whereas ALK-mediated lipid peroxidation contributed to the sensing step of NLRP3-NEK7 complex formation. PMID: 29723525
20. The role of NLGN3 in regulating depression in mice was confirmed in vitro using astrocytes stimulated by lipopolysaccharide (LPS) that NLGN3 knockdown reduced LPS-induced inflammation. PMID: 29775613
21. we found that butyrate significantly decreased Nlrp3 inflammasome formation and activation in the carotid arterial wall of wild type mice (Asc(+/+)), which was comparable to the effect of gene deletion of the adaptor protein apoptosis-associated speck-like protein gene (Asc(-/-)). PMID: 29475132
22. These results suggest that mitochondrial ROS-TXNIP/NLRP3/IL-1beta axis activation is responsible for tubular oxidative injury, which can be ameliorated by MitoQ via the inhibition of mtROS overproduction PMID: 29475133
23. Studied the anti-inflammatory effects of corylin on attenuation of lipopolysaccharide (LPS)-induced inflammation and inhibition of activation of nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasome in LPS-activated BV2 cells. PMID: 30107806
24. Data suggest that Abl2 on chromosome 1 and Nlrp3 on chromosome 11 are key to spermatozoa function; here, genetic polymorphisms in Abl2 and Nlrp3 appear to regulate susceptibility of mouse spermatozoa to cryopreservation. (Abl2 = v-abl Abelson murine leukemia viral oncogene 2; Nlrp3 = NLR family pyrin domain containing protein-3) PMID: 29269609
25. This study demonstrated that Hcy activates adipose NLRP3 inflammasomes in an adipocyte lyso-PC-dependent manner and highlights the importance of the adipocyte NLRP3 inflammasome in insulin resistance. PMID: 29735414
26. TLR2 and NLRP3 inflammasome activation in cardiac macrophages mediate the production of IL-1beta in diabetic mice. IL-1beta causes prolongation of the action potential duration, induces a decrease in potassium current and an increase in calcium sparks in cardiomyocytes, which are changes that underlie arrhythmia propensity. PMID: 27882934
27. TRIM31, through its E3 ubiquitin ligase activity, mediated K48-linked ubiqutination and proteasomal degradation of NLRP3. PMID: 27929086
28. fenofibrate attenuates oxidative stress and neuroinflammation in diabetic retinas, which is at least partially through modulating Nrf2 expression and NLRP3 inflammasome activation PMID: 29264825
29. NLRP3 inflammasome is inhibited by NSAIDs in mouse and rat models of Alzheimer's disease PMID: 27509875
30. These findings indicate that the NALP3 inflammasome is involved in H2O2induced type I collagen synthesis, which is mediated by the NFkappaB signaling pathway. Additionally, the NALP3 inflammasome contributes to the development of bleomycininduced pulmonary fibrosis. PMID: 29393339
31. 25-hydroxycholesterol contributes to cerebral inflammation of X-linked adrenoleukodystrophy through activation of the NLRP3 inflammasome. PMID: 27779191
32. Data suggest that gut microbiota present significantly altered growth patterns (that is, dysbiosis) in diabetic mice; these changes are attenuated in diabetic knockout mice lacking Nlrp3, an important component of inflammasomes and innate immunity. PMID: 29435463
33. Double-stranded RNA from M. tuberculosis induces NLRP3-dependent caspase-1 activation in retinal pigment epithelium. PMID: 29180292
34. NALP3 signaling is required for the induction of inflammatory response and the development of liver regeneration after PHx PMID: 28656530
35. this study reveals a novel differential role for NLRP3 inflammasome pathway in the immunity against clinically relevant Acinetobacter baumannii infections PMID: 28612844
36. Inhibition of NLRP3 inflammasome activation may be a potential therapeutic approach to alleviate MS-associated CNP and disease relapses in patients with RR-MS. PMID: 28965161
37. TREM-1 aggravates inflammation in acute lung injury by activating NLRP3 inflammasome, and blocking TREM-1 may be a potential therapeutic approach for acute lung injury PMID: 28004759
38. Findings indicate a selective vulnerability of sensory thalamic neurons in amyotrophic lateral sclerosis model mice, which is accompanied by massive dendrite swelling, accumulation of misfolded human SOD1, impaired autophagy and neuronal loss. Furthermore, ineffective autophagy and increased NLRP3 inflammasome expression in damaged neurons may be responsible for cell death and further spreading of the disease. PMID: 27880990
39. Data show that Pr2x7 gene deletion protects from HFD-induced NASH, possibly through blunted activation of NLRP3 inflammasome. PMID: 29270247
40. Results reveal a possible mechanism for AGE-induced pancreatic islet damage upon NLRP3 inflammasome activation. PMID: 29230270
41. Study demonstrated that activating alpha7nAChR contributed to the inhibition of NLRP3 inflammasome activation through the beta-arrestin-1-mediated manner in experimental auto-immune encephalomyelitis. PMID: 28941191
42. Results suggest a novel signaling pathway through which CB2 receptors are involved in the analgesic effect of electroacupuncture (EA) on inflammatory pain. These results suggest that EA reduces the inflammatory pain by inhibiting the activation of NLRP3 inflammasome through CB2 receptors. PMID: 28782714
43. miR-223 deficiency can lead to the sustained activation of NLRP3-IL-1beta. PMID: 29144508
44. NLRP3 plays a pivotal role in NASH development and pro-inflammatory cytokines IL-1beta and IL-18 secretion induced by PA stimulatio PMID: 28730512
45. Activation of Nlrp3 inflammasome may contribute to MPTP-induced neuroinflammation in SNpc, whereas miR-30e inhibits this process by targeting Nlrp3. PMID: 29274035
46. This study indicated that the NLRP3 inflammasome is significantly implicated in the progression of systemic inflammation-induced depression PMID: 29016824
47. findings provide compelling evidence of a role for Nlrp3 in nigral degeneration and neuroinflammation resulting from systemic rotenone exposure and suggest that the suppression of NLRP3 activity may be a rational neuroprotective strategy for toxin-associated PD PMID: 28903492
48. indicate the role of P2X7R (purinergic 2X7 receptor) and NLRP3 (NACHT, LRR, and PYD domains-containing protein 3) inflammasome activation in the process of pancreatic fibrosis in chronic pancreatitis (CP) and suggest that blockade of P2X7R-NLRP3 inflammasome signaling pathway may represent a therapeutic strategy for CP and its fibrotic process. PMID: 28930866
49. TLR4 and NLRP3 are suppressed by alpinectin, which attenuates inflammation in DSS-induced acute colitis PMID: 27321991
50. High expression of NLRP3 is associated with radiculopathy. PMID: 28404518

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Cytoplasm, cytosol. Inflammasome. Endoplasmic reticulum. Secreted. Nucleus.

NLRP family

Expressed with high levels in peripheral blood leukocytes, including Th2 lymphocytes and macrophages. Expressed at low levels in resting osteoblasts (at protein level).

KEGG: mmu:216799

STRING: 10090.ENSMUSP00000078440

UniGene: Mm.54174