Mouse N-myc-interactor(NMI) ELISA kit

中文名称：

小鼠N-Myc互作蛋白(NMI)酶联免疫试剂盒
货号：

CSB-EL015893MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
靶点详情

产品详情

产品描述：

小鼠N-Myc互作蛋白(NMI)酶联免疫试剂盒（CSB-EL015893MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NMI含量。小鼠N-Myc互作蛋白(NMI)是一种与NMYC和CMYC相互作用的蛋白质，参与细胞周期、凋亡和转化。NMI通过增强STAT介导的转录，在细胞因子IL2和IFN-γ的刺激下发挥作用，并在多种癌症中表达上调，可能成为癌症治疗的新靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，该产品适用于肿瘤生物学、免疫调控等基础研究领域，可应用于小鼠模型中的肿瘤微环境分析、免疫细胞功能研究或药物干预后NMI表达动态监测，为探索NMI在肿瘤抑制、炎症反应及细胞信号转导中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

NmiN-myc-interactor ELISA Kit; Nmi ELISA Kit; N-myc and STAT interactor ELISA Kit
缩写：

NMI
Uniprot No.：

O35309
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NMI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-102
1:2	Average %	92
	Range %	88-97
1:4	Average %	94
	Range %	85-99
1:8	Average %	100
	Range %	91-105

回收率：

The recovery of mouse NMI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	99	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.611	2.569	2.590	2.470
500	2.392	2.314	2.353	2.233
250	1.979	1.898	1.939	1.819
125	1.130	1.105	1.118	0.998
62.5	0.645	0.622	0.634	0.514
31.2	0.425	0.434	0.430	0.310
15.6	0.315	0.307	0.311	0.191
0	0.122	0.118	0.120

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

NMI and IFP35 serve as proinflammatory DAMPs during cellular infection and injury Xiahou Z.et al,Nat Commun.,2017

产品评价

靶点详情

功能：

Acts as a signaling pathway regulator involved in innate immune system response. In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude of signals for development and homeostasis. Enhances the recruitment of CBP/p300 coactivators to STAT1 and STAT5, resulting in increased STAT1- and STAT5-dependent transcription. In response to interferon IFN-alpha, associates in a complex with transcriptional regulator IFI35 to regulate immune response; the complex formation prevents proteasome-mediated degradation of IFI35. In complex with IFI35, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and transcription of NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-endothelialization of injured arteries. Negatively regulates virus-triggered type I interferon/IFN production by inducing proteosome-dependent degradation of IRF7, a transcriptional regulator of type I IFN, thereby interfering with cellular antiviral responses. Beside its role as an intracellular signaling pathway regulator, also functions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by macrophage to the extracellular space during cell injury or pathogen invasion. Macrophage-secreted NMI activates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 binding and activation, thereby inducing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines.
基因功能参考文献：
1. Damage-associated molecular patterns (DAMP) are important mediators of innate immunity. Here the authors show that N-myc and STAT interactor (NMI) and interferon-induced protein 35 (IFP35) act as DAMPs to promote inflammation by activating macrophages via the Toll-like receptor 4 and NF-kappaB pathways. PMID: 29038465
亚细胞定位：

Cytoplasm. Nucleus. Secreted.
蛋白家族：

NMI family
组织特异性：

Expressed in macrophages.
数据库链接：

KEGG: mmu:64685

STRING: 10090.ENSMUSP00000028314

UniGene: Mm.7491

Mouse N-myc-interactor(NMI) ELISA kit

产品详情

引用文献

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息