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  4. Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

Mouse Monocyte Chemotactic Protein 5 (MCP-5) ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠单核细胞趋化蛋白5(MCP-5)酶联免疫试剂盒
  • 货号：
    CSB-E15766m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠单核细胞趋化蛋白5（MCP-5）酶联免疫检测试剂盒（货号：CSB-E15766m），采用双抗体夹心法定量检测小鼠血清、血浆或组织匀浆样本中的MCP-5含量，检测范围为15.6 pg/mL至1000 pg/mL，最低检测限为15.6 pg/mL。MCP-5属于CC趋化因子家族，通过结合特定受体参与炎症反应调控、免疫细胞迁移等生物学过程，在感染性疾病、自身免疫病及肿瘤微环境研究中具有重要价值。本试剂盒基于高度特异性的配对抗体构建检测体系，通过预包被捕获抗体与样本中MCP-5结合，再经生物素标记检测抗体与酶标链霉亲和素系统放大信号，最终通过TMB显色反应实现目标蛋白的精准定量。适用于科研场景下对小鼠炎症相关模型、免疫调节机制或药物干预效果的研究，例如通过检测血清中MCP-5水平评估炎症程度，或分析组织匀浆中趋化因子表达变化以探究疾病发生机制，为免疫学、药理学及病理学研究提供可靠工具。
  • 别名：
    C-C motif chemokine 12 ELISA Kit; Ccl12 ELISA Kit; CCL12_MOUSE ELISA Kit; Macrophage/monocyte Chemotactic Protein ELISA Kit; MCP-1-related chemokine ELISA Kit; MCP-5 ELISA Kit; Monocyte chemoattractant protein 5 ELISA Kit; Monocyte chemotactic protein 5 ELISA Kit; OTTMUSP00000000792 ELISA Kit; Scya12 ELISA Kit; Small inducible cytokine A12 ELISA Kit; Small-inducible cytokine A12 ELISA Kit
  • 缩写：
    MCP-5
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    16.82 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 87    
    Range % 82-91    
    1:2 Average % 94    
    Range % 89-98    
    1:4 Average % 107    
    Range % 103-110    
    1:8 Average % 110    
    Range % 104-114    
  • 回收率：
    The recovery of mouse MCP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 91-99    
    EDTA plasma (n=4) 103 98-108    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    1000 2.615 2.634 2.625 2.499    
    500 2.177 2.012 2.095 1.969    
    250 1.432 1.502 1.467 1.341    
    125 0.842 0.839 0.841 0.715    
    62.5 0.531 0.529 0.530 0.404    
    31.2 0.334 0.326 0.330 0.204    
    15.6 0.246 0.251 0.249 0.123    
    0 0.125 0.127 0.126 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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靶点详情

  • 最新研究进展：
    MCP-5是一种趋化因子，全名为单核细胞趋化蛋白5。它主要参与免疫细胞的趋化和活化过程，在炎症、感染、肿瘤等疾病的发生和发展中发挥重要作用。最新研究发现，MCP-5参与了多种肿瘤的发生和进展，同时也是一种重要的治疗靶点。
  • 功能：
    Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
  • 基因功能参考文献：
    1. After 100% O2 treatment, genes involved in inflammation (Ccl12), angiogenesis (Igfr1, Stat3), and metabolism (Hk2) were upregulated. PMID: 24458145
    2. Data indicate mRNA upregulation of CCR2 and CCR2 ligands CCL2, CCL7, CCL8, and CCL12 in aortas from DOCA/salt-treated hypertension. PMID: 23033370
    3. The transforming growth factor (TGF)beta type II receptor/MCP-5 axis is an essential crossroad for joint development and endochondral growth. PMID: 22814601
    4. CCL12 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
  • 数据库链接：

    KEGG: mmu:20293

    STRING: 10090.ENSMUSP00000000194

    UniGene: Mm.867

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |