Mouse Membrane primary amine oxidase(AOC3) ELISA kit

Mouse Membrane primary amine oxidase(AOC3) ELISA kit

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中文名称：

小鼠伯胺氧化酶膜(AOC3)酶联免疫试剂盒
货号：

CSB-EL001855MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠伯胺氧化酶膜（AOC3）酶联免疫检测试剂盒（货号：CSB-EL001855MO），采用双抗体夹心法原理定量检测血清、血浆或组织匀浆样本中的AOC3蛋白水平，检测范围为1.25-80 ng/mL，最低检测限为1.25 ng/mL。实验通过预包被特异性抗体捕获样本中的靶蛋白，结合标记检测抗体形成复合物，经显色反应后通过标准曲线计算目标物浓度。AOC3作为一类表达于细胞膜表面的胺氧化酶，参与调控炎症反应、细胞黏附和免疫应答等过程，其表达水平与炎症性疾病、代谢紊乱及肿瘤微环境等研究密切相关。本试剂盒适用于科研场景下的小鼠模型研究，如通过检测血清或血浆样本评估循环系统中AOC3的动态变化，或通过组织匀浆分析特定器官（如肝脏、脂肪组织）中该蛋白的表达特征，为炎症机制、药物干预效果或免疫调节研究提供定量分析工具。试剂盒包含标准品、预包被板及检测所需试剂组分，操作便捷且特异性强。
别名：

Aoc3; Vap1; Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1
缩写：

AOC3
Uniprot No.：

O70423
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 ng/mL-80 ng/mL
灵敏度：

0.31 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	90
	Range %	85-96
1:4	Average %	96
	Range %	93-99
1:8	Average %	104
	Range %	100-109

回收率：

The recovery of mouse AOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	94-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
80	2.507	2.569	2.538	2.351
40	1.662	1.600	1.631	1.444
20	0.999	0.968	0.984	0.797
10	0.587	0.572	0.580	0.393
5	0.444	0.458	0.451	0.264
2.5	0.354	0.362	0.358	0.171
1.25	0.279	0.298	0.289	0.102
0	0.188	0.186	0.187

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
基因功能参考文献：
1. Data indicate strong-to-moderate expression of vascular adhesion protein-1 (VAP-1) in the synovium of Borrelia burgdorferi-infected mice, while only weak expression of VAP-1 was detected in uninfected mice. PMID: 29166944
2. VAP-1 was expressed on endothelial cells lining inflamed atherosclerotic lesions; normal vessel walls in aortas of C57BL/6N control mice were VAP-1-negative. PMID: 27731409
3. VAP-1 plays a critical role in the pathophysiology of renal ischemia/reperfusion injury by enhancement of neutrophil infiltration generating a local hydrogen peroxide gradient. PMID: 28318627
4. This study showed that the oxidase activity of AOC3 contributes to the development of lung fibrosis mainly by regulating the accumulation of pathogenic leukocyte subtypes, which drive the fibrotic response. PMID: 28251930
5. data indicate that SSAO inactivation in vivo stabilizes the established plaques mainly via inducing the switch of SMCs from a contractile to a synthetic phenotype PMID: 27043821
6. Suggest inhibiting VAP-1/SSAO enzymatic function, by PXS-4728A, as a novel therapeutic approach in lung diseases that are characterized by neutrophilic pattern of inflammation. PMID: 25889951
7. these results link the amine oxidase activity of VAP-1 with hepatic inflammation and fibrosis and suggest that targeting VAP-1 has therapeutic potential for NAFLD and other chronic fibrotic liver diseases. PMID: 25562318
8. these results suggest a largely redundant function for AOC3/VAP-1 in allergic inflammatory responses of the airways. PMID: 25116373
9. The results of the study establish VCAM-1 and VAP-1 as mediators of myeloid cell recruitment in metastasis and identify VAP-1 as a potential target for therapeutic intervention to combat early metastasis. PMID: 23407548
10. The antihyperglycemic effect of benzylamine is abolished by AOC3 gene knockout in mice. PMID: 22547093
11. Long-term activation of semicarbazide-sensitive amine oxidase by benzylamine lowers circulating levels of uric acid in diabetic conditions. PMID: 22480418
12. AOC3 expression is increased in perigonadal and subcutaneous adipose tissue of diabetic db-/- obese mice. PMID: 21298297
13. This review examines the biological functions of VAP-1, especially the role that this molecule might play in the establishment and progression of chronic liver disease. PMID: 21512782
14. The demonstrated AOC3 turnover of primary amines that are non-native to human tissue suggests possible roles for the adipocyte enzyme in subcutaneous bacterial infiltration and obesity. PMID: 22238597
15. VAP-1-mediated M2 macrophage infiltration underlies IL-1beta- but not VEGF-A-induced lymph- and angiogenesis PMID: 21435467
16. VAP-1 inhibition provided antiinflammation effect by reducing adhesion molecule expression and immune cell infiltration after intracerebral hemorrhage. PMID: 20877383
17. histamine moderately activated lipolysis in adipocytes in AOC3 knockout mice PMID: 20012150
18. Enzyme is in involved in deamination in atherogenesis in KKAy diabetic mice fed with high cholesterol diet. PMID: 12242458
19. the increased enzyme activity observed in diabetes is not a result of increased SSAO gene transcription. It is controlled by posttranslational modifications of the protein, and the catalytic activity controls the gene expression PMID: 12606817
20. vascular adhesion protein-1 expression may contribute to the functional heterogeneity of endothelial cells within the lung to create distinct sites for the recruitment of inflammatory cells PMID: 12700900
21. Release of this enzyme is regulated by TNF-alpha and insulin and, in adipose cells could contribute to the atherogenesis and vascular dysfunction associated with diabetes and obesity. PMID: 14968297
22. The vascular endothelium is the major source of circulating VAP-1 protein and semicarbazide-sensitive amine oxidase activity. PMID: 15178639
23. VAP-1 mediates leukocyte trafficking to sites of inflammation and thus is a potential target for anti-inflammatory therapies PMID: 15743791
24. T helper type 2 cells use Vap-1 to roll and adhere in the inflamed liver microcirculation PMID: 16111634
25. These data demonstrate the potential clinical benefit of small molecule anti-SSAO therapy in this mouse multiple sclerosis model. PMID: 17393060
26. The effects of exogenous or biogenic amines on glucose transport are not receptor-mediated but are oxidation-dependent. The major SSAO form expressed in mouse adipocytes is encoded by the AOC3 gene. PMID: 17406965
27. VAP-1-deficient mice have mild deviations in the mucosal immune system PMID: 17947691
28. The atheroprotective effect of LXR agonist T0901317 is related to the inhibition of SSAO gene expression and its activity. PMID: 18330481
29. LOX binds to mature TGF-beta1 and enzymatically regulates its signaling in bone via amine oxidase activity, and thus may play an important role in bone maintenance and remodeling PMID: 18835815
30. VAP-1 regulates the inflammatory response in ischemia-reperfusion injury and suggest that blockade of VAP-1 may have therapeutic value. PMID: 18991279
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亚细胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Copper/topaquinone oxidase family
数据库链接：

KEGG: mmu:11754

STRING: 10090.ENSMUSP00000099394

UniGene: Mm.67281

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.