Mouse Melanoma-derived growth regulatory protein(MIA) ELISA kit

Mouse Melanoma-derived growth regulatory protein(MIA) ELISA kit

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中文名称：

小鼠黑色素瘤源性生长调控蛋白(MIA)酶联免疫试剂盒
货号：

CSB-EL013798MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO小鼠黑色素瘤源性生长调控蛋白（MIA）酶联免疫检测试剂盒（货号：CSB-EL013798MO），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的MIA蛋白水平。MIA是一种由黑色素瘤细胞分泌的调控蛋白，通过影响细胞外基质黏附参与肿瘤侵袭与转移过程，是研究黑色素瘤进展的重要分子标记物。该试剂盒检测灵敏度为4.7 pg/mL，线性范围为4.7-300 pg/mL，通过预包被特异性抗体捕获目标蛋白，结合HRP标记检测抗体形成复合物，经显色反应后通过酶标仪测定吸光度值实现定量分析。适用于科研领域中对黑色素瘤动物模型的研究，如肿瘤微环境分析、转移机制探索、药物干预效果评估等场景，为揭示MIA在肿瘤生物学中的作用及开发相关治疗策略提供可靠工具。
别名：

Mia ELISA Kit; Cdrap ELISA Kit; Mia1 ELISA Kit; Melanoma-derived growth regulatory protein ELISA Kit; Cartilage-derived retinoic acid-sensitive protein ELISA Kit; CD-RAP ELISA Kit; Melanoma inhibitory activity protein ELISA Kit
缩写：

MIA
Uniprot No.：

Q61865
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.7 pg/mL-300 pg/mL
灵敏度：

1.17 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	82-99
1:200	Average %	101
	Range %	97-105
1:400	Average %	92
	Range %	88-96
1:800	Average %	87
	Range %	85-89

回收率：

The recovery of mouse MIA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-107
EDTA plasma (n=4)	96	92-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.502	2.563	2.533	2.426
150	2.256	2.212	2.234	2.127
75	1.698	1.680	1.689	1.582
37.5	0.859	0.899	0.879	0.772
18.75	0.514	0.536	0.525	0.418
9.4	0.301	0.326	0.314	0.207
4.7	0.192	0.172	0.182	0.075
0	0.107	0.106	0.107

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
基因功能参考文献：
1. The effects of MIA/CD-RAP on transcriptional regulation in chondrocytes, through the regulation of p54(nrb) via YBX1 contributes to the understanding of chondrogenesis. PMID: 24349210
2. Attenuated LM expressing MIA. PMID: 22886976
3. as observed in other knockout models of molecules important for cartilage development and differentiation, viability and functional integrity is reached by remarkable molecular redundancy in MIA/CD-RAP knockout mice. PMID: 24269712
4. The cartilage protein melanoma inhibitory activity contributes to inflammatory arthritis. PMID: 24287514
5. Expression analysis of cartilage tissue derived from MIA-/- mice revealed strong downregulation of nuclear RNA-binding protein 54-kDa. PMID: 21368873
6. MIA/CD-RAP stabilizes cartilage differentiation and inhibits differentiation into bone potentially by regulating signaling processes during differentiation. PMID: 20164682
7. transcriptional mechanism by which CD-RAP expression is suppressed by IL-1 beta PMID: 12072435
8. Cdrap/Mia is located between two housekeeping genes PMID: 15028289
9. DNA promoter segment from -2,251 bp to -2,068 bp of the CD-RAP gene contains elements critical for gene expression. It demonstrates activation or repression of gene expression in vitro and in vivo based on cell type and content of transcription factors. PMID: 16250001
10. A potential tumor suppressor role of Mia/Cd-rap in pituitary cells. PMID: 17914116
11. Microarray analysis showed significant reductions in the expression of several proteins in lung misexpressing MIA. PMID: 18342301
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亚细胞定位：

Secreted.
蛋白家族：

MIA/OTOR family
组织特异性：

All malignant melanoma cell lines tested and infrequently in glioma cell lines.
数据库链接：

KEGG: mmu:12587

UniGene: Mm.262447

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.