Mouse Mast Cell Tryptase(MCT) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠肥大细胞类胰蛋白酶(MCT)酶联免疫试剂盒（CSB-E14326m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MCT含量。MCT（单羧酸转运体）是重要的细胞膜转运蛋白。背景上，它在多种细胞代谢过程中起关键作用。研究机制方面，主要负责转运单羧酸类物质如乳酸等，在能量代谢、细胞内pH调节等过程有重要意义，其功能异常与肿瘤等疾病发展相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，广泛应用于科研领域，如肥大细胞活化机制研究、过敏性动物模型评估、炎症相关药物筛选等场景，为探索MCT在免疫调控中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Tpsab1 ELISA Kit; Mcpt7 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; mMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit
缩写：

MCT
Uniprot No.：

Q02844
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-96
1:2	Average %	96
	Range %	91-102
1:4	Average %	97
	Range %	93-100
1:8	Average %	93
	Range %	88-99

回收率：

The recovery of mouse MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-101
EDTA plasma (n=4)	91	84-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
基因功能参考文献：
1. transcriptional activation of mouse mast cell Protease-7 by activin and transforming growth factor-beta is inhibited by microphthalmia-associated transcription factor PMID: 14527958
2. Because mMCP-6 and mMCP-7 can compensate for each other in this mouse disease model, the elimination of both tryptases is necessary to reveal the prominent roles of these serine proteases in joint inflammation and destruction. PMID: 18668540
3. TGF-beta stimulates mmcp-7 transcription through the Smad3-Smad4 pathway as well as c-fos induction PMID: 16730810
蛋白家族：

Peptidase S1 family, Tryptase subfamily
数据库链接：

KEGG: mmu:100503895

STRING: 10090.ENSMUSP00000120741

UniGene: Mm.3301