Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

Mouse Lysosomal Pro-X carboxypeptidase(PRCP) ELISA kit

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中文名称：

小鼠溶酶体Pro-X羧肽酶(PRCP)酶联免疫试剂盒
货号：

CSB-EL018636MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠溶酶体Pro-X羧肽酶(PRCP)酶联免疫试剂盒（CSB-EL018636MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PRCP含量。小鼠溶酶体Pro-X羧肽酶（PRCP）是一种在溶酶体内发挥作用的蛋白酶，参与蛋白质的降解和代谢。其功能与调节血压和电解质平衡有关，研究其机制有助于理解高血压等疾病的发生。试剂盒检测范围为37.5 pg/mL-2400 pg/mL，适用于体外培养细胞模型中PRCP分泌调控研究、基因敲除动物模型的蛋白表达验证，或疾病状态下溶酶体功能相关分子机制的探索性分析，为心血管生物学、细胞代谢研究及溶酶体相关疾病机制研究提供可靠的定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Prcp ELISA Kit; Lysosomal Pro-X carboxypeptidase ELISA Kit; EC 3.4.16.2 ELISA Kit; Proline carboxypeptidase ELISA Kit; Prolylcarboxypeptidase ELISA Kit; PRCP ELISA Kit
缩写：

PRCP
Uniprot No.：

Q7TMR0
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

37.5 pg/mL-2400 pg/mL
灵敏度：

9.37 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	98
	Range %	92-103
1:4	Average %	93
	Range %	89-98
1:8	Average %	91
	Range %	87-96

回收率：

The recovery of mouse PRCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	94	93-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2400	2.542	2.520	2.531	2.377
1200	2.017	1.955	1.986	1.832
600	1.427	1.321	1.374	1.220
300	0.722	0.781	0.752	0.598
150	0.465	0.455	0.460	0.306
75	0.322	0.346	0.334	0.180
37.5	0.249	0.248	0.249	0.095
0	0.154	0.153	0.154

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
基因功能参考文献：
1. Prolylcarboxypeptidase (PRCP) converts AngII to Ang (1-7) but only at an acidic pH. Global PRCP deficiency causes heart and kidney alterations and a moderate rise in BP. PRCP is abundant in the kidney collecting tubules, where the prevailing pH is low. In collecting tubules, PRCP deficiency could result in impaired AngII degradation. Increased AngII at this nephron site stimulates Na reabsorption and increases BP. PMID: 28160049
2. activity and expression in plasma and lung tissue stays unaffected in lung ischemia-reperfusion injury PMID: 27437782
3. This study demonistrated that PRCP gene is broadly expressed in the brain. PMID: 24161824
4. PRCP regulates cell growth, angiogenesis, and the response to vascular injury PMID: 23744584
5. PRCP is an important regulator of energy and glucose homeostasis since its deletion significantly improves metabolic parameters in mice exposed to both standard chow diet and high-fat diet PMID: 22454290
6. analysis of non-benzimidazole and brain-penetrant prolylcarboxypeptidase inhibitors PMID: 22079761
7. This study showed that PRCP mutant mice have a significant decrease in fat mass, although an increase in lean mass was also observed; reduced leptin levels and increased energy expenditure were also found. PMID: 22202165
8. Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis. PMID: 21297000
9. PRCP is an important component of melanocortin signaling and weight maintenance via control of active alpha-MSH1-13 levels. PMID: 19620781
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亚细胞定位：

Lysosome.
蛋白家族：

Peptidase S28 family
数据库链接：

KEGG: mmu:72461

STRING: 10090.ENSMUSP00000075429

UniGene: Mm.389969

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.