Mouse Lipoxin A4,LXA4 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠脂氧素A4（LXA4）酶联免疫检测试剂盒（货号：CSB-E13268m），采用双抗体夹心法原理实现定量检测，适用于分析血清、血浆、细胞培养上清及组织匀浆等多种样本类型中的LXA4含量。LXA4作为重要的内源性脂质介质，在炎症消退过程中发挥关键调控作用，其动态变化与多种生理病理机制相关，是炎症性疾病研究的重要靶标。试剂盒检测灵敏度覆盖62.5 pg/mL至4000 pg/mL线性范围，实验操作流程包含预包被特异性捕获抗体的酶标板、生物素标记检测抗体及链霉亲和素-HRP系统，通过标准曲线可实现目标物精准定量。该试剂盒适用于体外实验环境中探究炎症反应调控机制、药物干预对脂氧素通路的影响，或评估疾病模型中LXA4的代谢动态，为炎症相关疾病基础研究、药物开发前效评价等提供可靠工具。其宽泛的样本兼容性与高特异性可满足复杂生物基质中低丰度LXA4的稳定检测需求。
缩写：

LXA4
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LXA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	98
	Range %	93-103
1:4	Average %	95
	Range %	90-100
1:8	Average %	94
	Range %	89-99

回收率：

The recovery of mouse LXA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	93	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.