Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse L-lactate dehydrogenase A chain(LDH-A)ELISA Kit

Mouse L-lactate dehydrogenase A chain(LDH-A)ELISA Kit

文献引用 (4) 说明书下载
  • 中文名称：
    小鼠L-乳酸脱氢酶A链(LDH-A)酶联免疫试剂盒
  • 货号：
    CSB-E17733m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠L-乳酸脱氢酶A链(LDH-A)酶联免疫试剂盒（CSB-E17733m）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDHA含量。LDHA是重要的靶点。它在细胞代谢尤其是糖酵解中发挥关键作用，与肿瘤细胞的能量供应和增殖密切相关。研究聚焦于其催化机制及在疾病发生发展中的作用，旨在开发能抑制其活性的药物，为肿瘤等疾病治疗提供新策略。试剂盒检测范围为0.156 mU/mL-10 mU/mL，该产品可广泛应用于肿瘤代谢重编程机制研究、缺血再灌注损伤模型中的乳酸代谢动态监测、运动生理学能量转换研究等领域，特别适用于比较不同处理条件下（如药物干预、基因敲除或过表达）样本中LDH-A活性的差异分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
  • 缩写：
    LDHA
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 mU/mL-10 mU/mL
  • 灵敏度：
    0.039 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 100
    Range % 95-106
    1:200 Average % 88
    Range % 84-93
    1:400 Average % 107
    Range % 103-110
    1:800 Average % 86
    Range % 83-90
  • 回收率：
    The recovery of mouse LDH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 96-108
    EDTA plasma (n=4) 85 81-90
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average
    10 0.144 0.148 0.146
    5 0.215 0.211 0.213
    2.5 0.325 0.331 0.328
    1.25 0.508 0.499 0.504
    0.625 0.763 0.778 0.771
    0.312 1.230 1.241 1.236
    0.156 1.561 1.570 1.566
    0 2.180 2.034 2.107
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

相关产品

Ldha Proteins

靶点详情

  • 基因功能参考文献：
    1. The release of lactate dehydrogenase A (LDHA) from degenerating neurons drives central nervous system (CNS) angiogenesis. PMID: 29248508
    2. Hair follicle stem cells maintain a metabolic state that allows them to remain dormant and yet quickly respond to appropriate proliferative stimuli mediated by LDH-A signaling. PMID: 28812580
    3. Results revealed that beta-arrestin-1 regulates lactate metabolism to contribute to beta2-adrenergic receptor functions in improved memory formation. PMID: 27916196
    4. The authors identified lactate dehydrogenase (LDH) as a new functional target of AMPKalpha1. PMID: 28515121
    5. LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and natural killer cells. PMID: 27641098
    6. lactate dehydrogenase A (LDHA) is induced in activated T cells to support aerobic glycolysis but promotes IFN-gamma expression independently of its 3'UTR. PMID: 27708054
    7. LDHA is a direct target of miR-34a in regulating glucose metabolism and tumor growth in breast cancer. PMID: 26902416
    8. Findings suggest that RANKL protein-induced lactate dehydrogenase (LDH) activation stimulates glycolytic and mitochondrial respiratory metabolism via transcription factor NFATc1 signaling. PMID: 27077737
    9. We propose a profibrotic feed forward loop, in which radiation induces LDHA expression and lactate production, which can lead to further activation of TGF-beta to drive the fibrotic process. PMID: 26254422
    10. High LDH-A expression is associated with metastases in breast tumors. PMID: 23833310
    11. PGC-1alpha reduces the expression of LDH A and one of its regulators, the transcription factor myelocytomatosis oncogene. PMID: 23650363
    12. Lactate dehydrogenase A is overexpressed in pancreatic cancer and promotes the growth of pancreatic cancer. PMID: 23404405
    13. Down-regulation of expression of stress-activated genes PKC-alpha, c-Myc and LDH-A by alpha-tocopherol in cancerous mice. PMID: 22132835
    14. The cumulus cells and oocytes showed intense signals for lactate dehydrogenase (LDH)-A respectively. PMID: 21360229
    15. isoproterenol induces changes in gastrocnemius muscle and serum lactate dehydrogenase expression in mice PMID: 21552790
    16. Cocaine could inhibit the activities of ATPase, LDH and SDH in cultured splenocytes. PMID: 20653129
    17. The increased LDH-A/LDH-B ratio causes high brain lactate levels, which, in turn, are predictive of aging phenotypes. PMID: 21041631
    18. The muscle subunit of lactate dehydrogenase is an integral part of the sarcolemmal K(ATP) channel complex in vivo, where it couples the metabolic status of the cell with the K(ATP) channel activity essential for protection against ischemia. PMID: 12145195
    19. Reduction in LDH-A activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. The tumorigenicity of the LDH-A-deficient cells was severely diminished. PMID: 16766262
    20. beta-agonists increased serum LDH levels, but it was not due to release from the heart. PMID: 17853776
    21. M-LDH released from cardiomyocytes after hypoxia and reoxygenation has a role in protecting the heart from oxidative stress-induced injury through an intracellular signal transduction pathway involving ERK1/2. PMID: 18534967

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    LDH/MDH superfamily, LDH family
  • 数据库链接：

    KEGG: mmu:16828

    STRING: 10090.ENSMUSP00000103267

    UniGene: Mm.29324

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |