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  4. Mouse L-amino-acid oxidase(IL4I1) ELISA kit

Mouse L-amino-acid oxidase(IL4I1) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    小鼠L氨基酸氧化酶(IL4I1)酶联免疫试剂盒
  • 货号：
    CSB-EL011660MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠L-氨基酸氧化酶（IL4I1）酶联免疫检测试剂盒（货号：CSB-EL011660MO），采用夹心法原理实现定量分析，适用于检测血清、血浆、组织匀浆及细胞裂解液等多种样本类型，检测灵敏度范围为15.6-1000 pg/mL。IL4I1是一种具有免疫调节功能的酶，通过催化L-氨基酸氧化反应参与肿瘤微环境调控、T细胞功能抑制等生物学过程，近年来在免疫代谢和疾病机制研究中备受关注。该试剂盒通过高特异性抗体对靶标进行双位点捕获，可精准检测样本中IL4I1的浓度变化，线性范围覆盖常见实验需求，操作流程标准化且重复性良好。科研人员可将其应用于免疫学基础研究、肿瘤模型构建、炎症性疾病机制探索等领域，例如评估不同病理状态下IL4I1在血液或组织中的表达水平，或通过体外细胞实验分析其功能调控网络，为揭示IL4I1在免疫代谢中的分子机制提供可靠工具。试剂盒配套提供标准品及预包被酶标板，支持实验室灵活开展小规模或高通量检测。
  • 别名：
    Il4i1 ELISA Kit; Fig1 ELISA Kit; L-amino-acid oxidase ELISA Kit; LAAO ELISA Kit; LAO ELISA Kit; EC 1.4.3.2 ELISA Kit; Interleukin-4-induced protein 1 ELISA Kit; IL4-induced protein 1 ELISA Kit; Protein Fig-1 ELISA Kit; mFIG1 ELISA Kit
  • 缩写：
    IL4I1
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL4I1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 101
    Range % 92-105
    1:2 Average % 94
    Range % 90-99
    1:4 Average % 95
    Range % 87-98
    1:8 Average % 92
    Range % 85-95
  • 回收率：
    The recovery of mouse IL4I1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 103 94-107
    EDTA plasma (n=4) 98 90-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 1.780 1.814 1.797 1.596
    500 1.316 1.301 1.309 1.108
    250 0.954 0.939 0.947 0.746
    125 0.716 0.732 0.724 0.523
    62.5 0.488 0.498 0.493 0.292
    31.2 0.406 0.400 0.403 0.202
    15.6 0.306 0.294 0.300 0.099
    0 0.203 0.199 0.201 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Il4i1 Proteins

靶点详情

  • 功能：
    Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cell, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2.
  • 基因功能参考文献：
    1. Data showed proteome changes in alveolar epithelial cells type II exposed to an infection with A. fumigatus with IL4I1 showing the most prominent increase in abundance both, in terms of relative abundance as well as activity. Also, in the infected lungs, there was levels of IL4I1 metabolic products. PMID: 28951444
  • 亚细胞定位：
    Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome.; [Isoform 1]: Lysosome.; [Isoform 2]: Lysosome.
  • 蛋白家族：
    Flavin monoamine oxidase family, FIG1 subfamily
  • 组织特异性：
    Primarily found in immune tissues.; [Isoform 1]: Primarily found in immune tissues, mostly in B-lymphocytes.; [Isoform 2]: Restricted to the testis, predominantly in Sertoli cells at the periphery of the ducts, and the brain, including Purkinje cells, hip
  • 数据库链接：

    KEGG: mmu:100328588

    STRING: 10090.ENSMUSP00000113726

    UniGene: Mm.2565

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |