Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠细胞角蛋白18片段Asp396(K18-Asp396)酶联免疫试剂盒（CSB-E14265m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK 18-M30含量。CK 18-M30是细胞角蛋白18的一个片段。它与细胞凋亡相关，在细胞凋亡时，特定的酶会切割CK18产生M30。在医学研究中，可作为细胞凋亡的生物标志物，用于多种疾病如肝脏疾病、肿瘤等的诊断、病情监测和预后评估。试剂盒检测范围为15.6 μIU/mL-1000 μIU/mL，该产品可广泛应用于科研领域，例如评估药物或毒素诱导的肝细胞凋亡模型、上皮源性肿瘤细胞死亡机制研究，或炎症性疾病中上皮屏障功能损伤的定量分析，为体外实验提供稳定的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CK 18-M30
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 μIU/mL-1000 μIU/mL
灵敏度：

3.9 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
Range %	89-104
1:200	Average %	95
Range %	90-100
1:400	Average %	92
Range %	87-101
1:800	Average %	93
Range %	90-97

回收率：

The recovery of mouse CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	96	90-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.