Mouse Interleukin 35(IL-35) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为科研领域设计的小鼠白介素35（IL-35）定量检测试剂盒（货号：CSB-E13145m），采用双抗体夹心法ELISA原理，可精准检测血清、血浆、细胞培养上清及组织匀浆等多种样本中IL-35的表达水平。IL-35作为白介素12家族成员，主要由调节性T细胞分泌，在免疫耐受建立和炎症反应调控中发挥关键作用，其表达水平与自身免疫性疾病、慢性炎症及肿瘤微环境等研究密切相关。试剂盒具备15.6-1000 pg/mL的宽检测范围，最低检测限达7.8 pg/mL，可灵敏捕捉低丰度目标分子，适用于体外培养细胞因子分泌分析、实验动物模型血清/组织IL-35动态监测等场景。通过预包被高亲和力捕获抗体与标记检测抗体的协同作用，配合标准化的显色系统，确保实验数据的可靠性与重复性。该试剂盒为研究IL-35在免疫调控机制、疾病模型中的功能解析及药物干预效果评价提供了高效工具，广泛应用于免疫学、肿瘤学及炎症相关基础研究领域。
缩写：

IL-35
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-97
1:2	Average %	95
	Range %	89-99
1:4	Average %	94
	Range %	90-100
1:8	Average %	93
	Range %	87-97

回收率：

The recovery of mouse IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	96	91-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.