Mouse Interferon epsilon(IFNE) ELISA kit

产品详情

产品描述：

小鼠干扰素ε(IFNE)酶联免疫试剂盒（CSB-EL011049MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNE含量。IFNE 即Ⅰ型干扰素，它在固有免疫和适应性免疫中有关键作用。研究发现，IFNE 能调节免疫细胞活性、增强抗病毒能力，还可参与肿瘤免疫监视。其作用机制包括激活相关信号通路，诱导抗病毒蛋白产生，抑制病毒复制与肿瘤细胞生长。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于基础科研中探究IFN - ε在生殖生物学、免疫调控机制或感染性疾病模型中的作用，如卵巢/子宫组织样本分析、妊娠相关体液因子检测或黏膜免疫系统功能研究。数据结果可用于分子机制研究或生物标志物筛选，但不适用于工业生产或药物开发。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Ifne ELISA Kit; Ifne1 ELISA Kit; Ifnt1Interferon epsilon ELISA Kit; IFN-epsilon ELISA Kit; Interferon epsilon-1 ELISA Kit; Interferon tau-1 ELISA Kit
缩写：

IFNE
Uniprot No.：

Q80ZF2
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-88
1:2	Average %	94
	Range %	89-98
1:4	Average %	103
	Range %	97-108
1:8	Average %	99
	Range %	95-104

回收率：

The recovery of mouse IFNE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
EDTA plasma (n=4)	89	83-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Type I interferon required for maintaining basal levels of IFN-regulated genes, including 2'-5'-oligoadenylate synthetase, IRF7 and ISG15, in the female reproductive tract. Directly mediates protection against viral, including HSV-2, and bacterial, including Chlamydia muridarum, genital infections.
基因功能参考文献：
1. IFN-epsilon is constitutively expressed by cells of the reproductive tract and is inefficiently secreted by fibroblasts and cell lines. PMID: 23951133
2. IFN-epsilon could promote migration of antigen-specific CD8(+)T cells to the gut. PMID: 22617838
3. Ifn-epsilon-deficient mice had increased susceptibility to infection of the female reproductive tract by the common sexually transmitted infections (STIs) herpes simplex virus 2 and Chlamydia muridarum. PMID: 23449591
4. interactions between estradiol and IFN-tau in the context of the overall immune response during autoimmune orchitis PMID: 21787220
亚细胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
组织特异性：

Expressed at very high levels in uterus and, at much lower levels, in ovary and cervix. Very low levels, if any, in other organs. In the endometrium, expressed in the luminal and glandular epithelial cells (at protein level).
数据库链接：

KEGG: mmu:230405

STRING: 10090.ENSMUSP00000059199

UniGene: Mm.246593