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  4. Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit

Mouse Integrin alpha-M (Itgam/CD11b) ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠整合素αM(Itgam/CD11b)酶联免疫试剂盒
  • 货号：
    CSB-E17417m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO小鼠整合素αM（Itgam/CD11b）酶联免疫吸附检测试剂盒（货号：CSB-E17417m），采用双抗体夹心法原理设计，可对血清、血浆、细胞培养上清及组织匀浆样本中的Itgam/CD11b进行定量检测，检测灵敏度为15.6 pg/mL，线性范围为15.6-1000 pg/mL，适用于不同浓度样本的分析。整合素αM作为白细胞表面重要的黏附分子，与CD18组成Mac-1复合物，参与细胞迁移、吞噬作用和炎症反应调控，是免疫学研究的关键靶点。该试剂盒通过包被特异性捕获抗体和标记检测抗体的协同作用，实现对目标蛋白的高特异性识别，可广泛应用于科研领域中小鼠免疫细胞功能研究、炎症性疾病模型的分子机制探索、药物干预实验的效应评估等场景，特别适用于巨噬细胞、中性粒细胞等髓系细胞在感染、自身免疫病或肿瘤微环境中的Itgam/CD11b表达水平分析，为免疫调控机制研究提供可靠工具。
  • 别名：
    Itgam ELISA Kit; Integrin alpha-M ELISA Kit; CD11 antigen-like family member B ELISA Kit; CR-3 alpha chain ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; CD antigen CD11b ELISA Kit
  • 缩写：
    Itgam/CD11b
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Itgam/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 86
    Range % 81-90
    1:200 Average % 103
    Range % 99-108
    1:400 Average % 97
    Range % 92-101
    1:800 Average % 90
    Range % 86-94
  • 回收率：
    The recovery of mouse Itgam/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 88 84-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.603 2.527 2.565 2.376
    500 1.920 1.865 1.893 1.704
    250 1.367 1.441 1.404 1.215
    125 0.905 0.928 0.917 0.728
    62.5 0.586 0.574 0.580 0.391
    31.2 0.439 0.419 0.429 0.240
    15.6 0.291 0.304 0.298 0.109
    0 0.186 0.192 0.189 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Itgam Proteins

靶点详情

  • 功能：
    Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development.
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
  • 蛋白家族：
    Integrin alpha chain family
  • 组织特异性：
    Predominantly expressed in monocytes and granulocytes. Expressed in a subset of peritoneal mast cells. Expressed in microglia (at protein level).
  • 数据库链接：

    STRING: 10090.ENSMUSP00000101849

    UniGene: Mm.262106

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |