Mouse Insulin-like growth factor-binding protein complex acid labile subunit(IGFALS) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠胰岛素样生长因子结合蛋白复合物酸不稳定亚基(IGFALS)酶联免疫试剂盒（CSB-EL011094MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGFALS含量。IGFALS即胰岛素样生长因子结合蛋白酸不稳定亚基，是胰岛素样生长因子（IGF）轴的关键组分，能与IGF和结合蛋白形成三元复合物，延长IGF半衰期。目前对其研究主要聚焦于在生长发育、代谢及疾病如肿瘤中的作用机制。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于探索小鼠模型中IGFALS在生长发育异常、代谢性疾病、内分泌调控及相关信号通路中的功能机制，也可用于评估药物或实验干预对IGF系统的影响，为相关基础研究提供可靠的定量分析工具，满足科研场景下对IGFALS表达水平的动态监测需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Igfals ELISA Kit; Albs ELISA Kit; AlsInsulin-like growth factor-binding protein complex acid labile subunit ELISA Kit; ALS ELISA Kit
缩写：

IGFALS
Uniprot No.：

P70389
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFALS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	93-99
1:200	Average %	84
	Range %	80-87
1:400	Average %	91
	Range %	87-94
1:800	Average %	107
	Range %	104-110

回收率：

The recovery of mouse IGFALS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	85	81-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

May have an important role in regulating the access of circulating IGFs to the tissues.
基因功能参考文献：
1. the Igfals gene has a role in skeletal response of male mice to anabolic hormone therapy PMID: 24424061
2. A modest reduction in post-natal growth in the null ALS mice and in the ALS-deficient patients was observed PMID: 16114275
亚细胞定位：

Secreted, extracellular space.
数据库链接：

KEGG: mmu:16005

STRING: 10090.ENSMUSP00000060169

UniGene: Mm.3135