Mouse Hydroxyacid oxidase 1(HAO1) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠羟基酸氧化酶1(HAO1)酶联免疫试剂盒（CSB-EL010127MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HAO1含量。小鼠羟基酸氧化酶1（HAO1）是草酸代谢中的关键酶，其表达下调可显著降低细胞草酸生成，具有作为干预细胞草酸生成靶点的潜力。研究机制涉及HAO1在氧化应激损伤中的作用，以及其对相关疾病如糖尿病和肿瘤转移的影响。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于探索HAO1在代谢通路中的分子机制、评估基因敲除/过表达模型小鼠的酶活性变化，或研究药物干预、营养状态等因素对HAO1表达的影响，为脂质代谢相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Hao1 ELISA Kit; Gox1 ELISA Kit; Hao-1 ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; HAOX1 ELISA Kit; EC 1.1.3.15 ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit
缩写：

HAO1
Uniprot No.：

Q9WU19
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	83-107
1:2	Average %	95
	Range %	89-103
1:4	Average %	92
	Range %	85-105
1:8	Average %	91
	Range %	87-99

回收率：

The recovery of mouse HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-103
EDTA plasma (n=4)	96	90-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
亚细胞定位：

Peroxisome.
蛋白家族：

FMN-dependent alpha-hydroxy acid dehydrogenase family
组织特异性：

Liver.
数据库链接：

KEGG: mmu:15112

STRING: 10090.ENSMUSP00000028704

UniGene: Mm.26634