Mouse Glutaredoxin-related protein 5, mitochondrial(GLRX5) ELISA kit

产品详情

产品描述：

小鼠谷胱甘肽相关蛋白5，线粒体(GLRX5)酶联免疫试剂盒（CSB-EL009524MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GLRX5含量。GLRX5是一个有研究价值的靶点。它在人体内参与铁硫簇的生物合成等重要生理过程。其异常可能与多种疾病相关，如血液系统疾病等。目前研究聚焦于其在疾病发生发展机制中的作用，以探寻基于此靶点的潜在治疗策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探究GLRX5在氧化应激、线粒体功能障碍或铁代谢异常等病理生理模型中的表达变化，例如通过检测肝、脑等组织或培养细胞中的蛋白水平，揭示其在神经退行性疾病、代谢综合征或贫血相关研究中的作用机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Glrx5Glutaredoxin-related protein 5 ELISA Kit; mitochondrial ELISA Kit; Monothiol glutaredoxin-5 ELISA Kit
缩写：

GLRX5
Uniprot No.：

Q80Y14
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GLRX5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-96
1:2	Average %	96
	Range %	92-100
1:4	Average %	84
	Range %	80-88
1:8	Average %	93
	Range %	88-97

回收率：

The recovery of mouse GLRX5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	106	102-113

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer. Receives 2Fe/2S clusters from scaffold protein ISCU and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1.
基因功能参考文献：
1. Study reports a decrease of Grx expression levels in pancreatic islets of diabetic mice which was accompanied by declining insulin secretion, increase of reactive oxygen species (ROS) production level, and cell cycle alterations. These data demonstrate the essential role of the Grx system for the beta-cell during metabolic stress which may provide a new target for diabetes mellitus type 2 treatment. PMID: 28542222
2. Grx5 is an important determinant of osteoblast apoptosis and acts via a molecular pathway that involves regulation of ROS production, cardiolipin oxidation, caspase activity, Fe-S cluster formation, and MnSOD activity. PMID: 19442627
亚细胞定位：

Mitochondrion matrix.
蛋白家族：

Glutaredoxin family, Monothiol subfamily
组织特异性：

Detected in bone, liver, muscle and kidney.
数据库链接：

KEGG: mmu:73046

STRING: 10090.ENSMUSP00000021522

UniGene: Mm.29128