Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Mouse Glia-derived nexin(SERPINE2) ELISA kit

Mouse Glia-derived nexin(SERPINE2) ELISA kit

说明书下载
  • 中文名称：
    小鼠胶质细胞源性连接蛋白(SERPINE2)酶联免疫试剂盒
  • 货号：
    CSB-EL021082MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠胶质细胞源性连接蛋白(SERPINE2)酶联免疫试剂盒（CSB-EL021082MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SERPINE2含量。SERPINE2是重要靶点。它在细胞生长、迁移、黏附及组织重塑等生理过程有作用。研究机制方面，涉及调节蛋白酶活性等，其异常表达与肿瘤、神经系统疾病等病理状况相关，深入研究能为相关疾病治疗提供新方向。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品可广泛应用于神经退行性疾病模型（如阿尔茨海默病）、中枢神经损伤修复机制、胶质瘤微环境研究等科研领域，特别适用于探究脑卒中、脊髓损伤等病理过程中胶质细胞功能动态变化，以及肿瘤微血管生成相关分子机制分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Serpine2 ELISA Kit; Pi7 ELISA Kit; Pn1 ELISA Kit; Spi4Glia-derived nexin ELISA Kit; GDN ELISA Kit; Peptidase inhibitor 7 ELISA Kit; PI-7 ELISA Kit; Protease nexin 1 ELISA Kit; PN-1 ELISA Kit; Protease nexin I ELISA Kit; Serine protease-inhibitor 4 ELISA Kit; Serpin E2 ELISA Kit
  • 缩写：
    SERPINE2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPINE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 103
    Range % 99-112
    1:200 Average % 84
    Range % 80-88
    1:400 Average % 97
    Range % 92-101
    1:800 Average % 90
    Range % 85-94
  • 回收率：
    The recovery of mouse SERPINE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 85-93
    EDTA plasma (n=4) 95 90-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    200 2.185 2.312 2.249 2.111
    100 1.841 1.937 1.889 1.751
    50 1.437 1.385 1.411 1.273
    25 0.946 1.002 0.974 0.836
    12.5 0.598 0.579 0.589 0.451
    6.25 0.362 0.374 0.368 0.230
    3.12 0.235 0.226 0.231 0.093
    0 0.134 0.141 0.138 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.
  • 基因功能参考文献：
    1. SerpinE2 is required in the extracellular milieu of breast tumors where it acts in multiple ways to regulate tumor matrix deposition, thereby controlling tumor cell dissemination. PMID: 27793045
    2. These data uncover a novel mechanism whereby loss of serine protease inhibition leads to lung lymphocyte accumulation. PMID: 27059719
    3. the involvement of PAI-1 in HIF-1-enhanced thrombosis and that an additional factor participates in regulating Pai-1 expression in compressed skin. PMID: 25939592
    4. aberrant Serpine2/Pn-1 is required for proliferation of human and mouse medulloblastoma cells. PMID: 25901736
    5. PN-1 strongly limits physiological angiogenesis. PMID: 26109427
    6. The PAI-1 inhibitor not only accelerated the expansion of the donor HSCs during the early-stage of regeneration, but also supported long-term hematopoiesis. PMID: 24155177
    7. a novel inhibitory interaction between CAP1/Prss8 and nexin-1, opening the search for specific CAP1/Prss8 antagonists that are independent of its catalytic activity PMID: 25138159
    8. SERPINE2 and PLAU are involved in cumulus expansion and oocyte maturation. PMID: 24023701
    9. Endothelial protease nexin-1 is a novel regulator of A disintegrin and metalloproteinase 17 maturation and endothelial protein C receptor shedding via furin inhibition. PMID: 23661674
    10. PN-1 has direct antiangiogenic properties and is a yet-unrecognized player in the angiogenic balance PMID: 22331468
    11. SERPINE2 protein may play a role as a sperm decapacitation factor. PMID: 21084713
    12. platelet PN-1 can be considered as a new important regulator of thrombolysis in vivo PMID: 21403095
    13. SERPINE2 is a major plasminogen activator(PA) inhibitor in the placenta and uterus during the estrous cycle, pregnancy, and lactation. It may participate in the PA-modulated tissue remodeling process in the mouse placenta and uterus. PMID: 20977773
    14. Data suggest that lack of extinction in protease nexin-1 KO mice is associated with changes in neuronal activity in the circuitry of the basal and central amygdaloid nuclei, supporting a differential impact on fear extinction in these areas. PMID: 20529116
    15. Platelet PN-1 is a key player in the thrombotic process, whose negative regulatory role has been, up to now, markedly underestimated. PMID: 19855083
    16. PN-1 may have a role in regulating activities of muscle proteases PMID: 14733957
    17. We report that brain proteolytic activity was strongly regulated by neuronal activity, with PN-1 being a major controlling element. PMID: 15509762
    18. p65 binds to the kappaB-like DNA sequence located at approximately -9 kbp in the SerpinE2 PMID: 16697375
    19. Expression array analysis in murine models demonstrated association and repication of SERPINE2 as a potential COPD susceptibility gene. PMID: 16921128
    20. Results indicate that PN-1 could have a natriuretic role by inhibiting prostasin activity. Aldosterone and TGF-beta reciprocally regulate the expression of PN-1. PN-1 could represent a new factor that contributes to regulation of ENaC activity. PMID: 16941024
    21. Schwann cell-derived protease nexin-1 is crucial for proper reinervation and cell survival. PMID: 17409231
    22. PN-1 is upregulated in cryoglobulinemic membranoproliferative glomerulonephritis PMID: 18199802
    23. Results suggest that PN-1 might become a prognostic marker in breast cancer. PMID: 19584287

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Serpin family
  • 组织特异性：
    Most abundant in seminal vesicles.
  • 数据库链接：

    KEGG: mmu:20720

    STRING: 10090.ENSMUSP00000027467

    UniGene: Mm.3093

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |