Mouse Galectin 3(GAL-3) ELISA Kit

Mouse Galectin 3(GAL-3) ELISA Kit

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中文名称：

小鼠半乳糖凝集素-3(Gal-3)酶联免疫试剂盒
货号：

CSB-E14296m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠半乳糖凝集素-3（Gal-3）酶联免疫检测试剂盒（货号CSB-E14296m），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的Gal-3蛋白水平。Gal-3作为β-半乳糖苷结合凝集素家族成员，广泛参与细胞增殖、凋亡及免疫调节过程，其异常表达与炎症反应、器官纤维化及肿瘤进展密切相关。本试剂盒检测灵敏度达0.312 ng/mL，线性范围覆盖20 ng/mL，可精准量化低丰度样本中的靶蛋白含量，适用于小鼠疾病模型构建、药物干预研究及分子机制探索等科研领域，特别针对心血管疾病、肝纤维化或肿瘤微环境研究中Gal-3的动态变化监测。试剂盒包含预包被捕获抗体、生物素标记检测抗体及标准品等核心组分，操作流程经优化可在4.5小时内完成，配套的显色系统通过酶标仪读取450 nm吸光度值实现定量分析，为科研人员提供稳定可靠的检测方案。
别名：

Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
缩写：

LGALS3
Uniprot No.：

P16110
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-103
1:2	Average %	90
	Range %	82-99
1:4	Average %	92
	Range %	86-100
1:8	Average %	92
	Range %	89-103

回收率：

The recovery of mouse GAL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	97	91-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.852	2.842	2.847	2.703
10	2.207	2.280	2.244	2.100
5	1.357	1.384	1.371	1.227
2.5	0.841	0.862	0.852	0.708
1.25	0.475	0.458	0.467	0.323
0.625	0.283	0.297	0.290	0.146
0.312	0.231	0.244	0.238	0.094
0	0.142	0.146	0.144

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Galectin-3 Inhibition Ameliorates Alveolar Epithelial Cell Pyroptosis in Phosgene-Induced Acute Lung Injury O Memet,/,/
Noni (Morinda citrifolia L.) Fruit Polysaccharide Attenuated Inflammatory Bowel Disease Mice: Association of STAT3/NLRP3 Signaling Pathways MY Jin,/,2023
Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization H Liu,iScience,2023
Evaluation of Annexin A7, Galectin-3 and Gelsolin as possible biomarkers of hepatocarcinoma lymphatic metastasis Wang X et al,Biomed Pharmacother,2014

功能：

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
亚细胞定位：

Cytoplasm. Nucleus. Secreted.
组织特异性：

The highest levels are found in activated macrophages.
数据库链接：

STRING: 10090.ENSMUSP00000114350

UniGene: Mm.248615

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.