Mouse GDNF family receptor alpha-like(GFRAL) ELISA kit

产品详情

产品描述：

CUSABIO小鼠GDNF家族α样受体（GFRAL）酶联免疫检测试剂盒（货号CSB-EL009383MO），采用双抗体夹心酶联免疫吸附法定量分析血清、血浆、组织匀浆及细胞裂解液样本中GFRAL的蛋白表达水平，可检测浓度范围为25 pg/mL至1600 pg/mL。GFRAL作为胶质细胞源性神经营养因子（GDNF）家族的受体成员，近年研究显示其与能量代谢调控、食欲中枢信号传导及糖脂代谢平衡密切相关，尤其在肥胖和糖尿病相关机制研究中具有重要价值。本试剂盒通过高特异性配对抗体实现目标蛋白的精准捕获与信号放大，适用于基础科研领域探索GFRAL在代谢性疾病动物模型中的动态变化、评估特定组织或细胞中受体表达差异，以及筛选调控GFRAL通路的药物或生物活性分子。产品严格验证了线性范围与灵敏度指标，可满足体外实验中低丰度蛋白的稳定检测需求，为代谢机制研究、神经内分泌调控及疾病模型构建提供可靠工具。
别名：

Gfral ELISA Kit; Gral ELISA Kit; GDNF family receptor alpha-like ELISA Kit
缩写：

GFRAL
Uniprot No.：

Q6SJE0
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFRAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-98
1:2	Average %	101
	Range %	97-107
1:4	Average %	94
	Range %	90-98
1:8	Average %	97
	Range %	92-101

回收率：

The recovery of mouse GFRAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	88	84-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
基因功能参考文献：
1. isolating GFRAL as the receptor for GDF15-induced anorexia and weight loss, the identification of a mechanistic basis for the non-homeostatic regulation of neural circuitry by a peripheral signal associated with tissue damage and stress; findings provide opportunities to develop therapeutic agents for the treatment of disorders with altered energy demand PMID: 28953886
亚细胞定位：

Cell membrane; Single-pass membrane protein; Extracellular side.
蛋白家族：

GDNFR family
组织特异性：

Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius.
数据库链接：

KEGG: mmu:404194

UniGene: Mm.213862