Mouse Filaggrin(FLG) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠丝聚蛋白（Filaggrin, FLG）酶联免疫检测试剂盒（货号：CSB-EL008712MO），采用双抗体夹心法定量分析血清、血浆、组织匀浆及细胞裂解液等样本中的FLG含量，检测灵敏度为3.12 pg/mL，线性范围覆盖3.12-200 pg/mL。FLG是表皮颗粒层的重要结构蛋白，参与皮肤屏障的形成与角质化过程，其表达异常可能与皮肤干燥、炎症性疾病或遗传性皮肤病相关。本试剂盒通过高特异性抗体实现精准检测，适用于科研领域探索小鼠模型中FLG的表达调控机制，如研究皮肤屏障功能障碍、特应性皮炎等疾病的分子机制，或评估药物干预、基因修饰对FLG蛋白水平的影响。实验操作便捷，可高效兼容多种生物样本类型，为体外实验或动物模型研究提供可靠工具。
别名：

FlgFilaggrin ELISA Kit; Fragment ELISA Kit
缩写：

FLG
Uniprot No.：

P11088
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FLG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	92-101
1:4	Average %	88
	Range %	86-95
1:8	Average %	90
	Range %	86-95

回收率：

The recovery of mouse FLG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	101	95-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
亚细胞定位：

Cytoplasmic granule.
蛋白家族：

S100-fused protein family
组织特异性：

Expressed in the granular layer of the epidermis.
数据库链接：

STRING: 10090.ENSMUSP00000054773

UniGene: Mm.162684