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  4. Mouse Ficolin-2(FCN2) ELISA kit

Mouse Ficolin-2(FCN2) ELISA kit

说明书下载
  • 中文名称：
    小鼠纤维胶凝蛋白-2(FCN2)酶联免疫试剂盒
  • 货号：
    CSB-EL008551MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠纤维胶凝蛋白-2(FCN2)酶联免疫试剂盒（CSB-EL008551MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FCN2含量。小鼠纤维胶凝蛋白-2（FCN2）是一种具有唾液酸结合活性的蛋白，参与补体激活和凝集素途径，在天然免疫应答中发挥重要作用。其功能与血浆膜外成分和胶原三聚体相关，主要在骨髓、胚胎、肝脏和脾脏中表达。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于免疫调节机制、感染性疾病模型及炎症相关研究，例如探索病原体感染、自身免疫性疾病或代谢紊乱等实验条件下FCN2的动态表达特征，为小鼠模型中免疫微环境变化提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Fcn2 ELISA Kit; FcnbFicolin-2 ELISA Kit; Collagen/fibrinogen domain-containing protein 2 ELISA Kit; Ficolin-B ELISA Kit; Ficolin-beta ELISA Kit; L-ficolin ELISA Kit
  • 缩写：
    FCN2
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FCN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 92
    Range % 88-96
    1:2000 Average % 94
    Range % 90-98
    1:4000 Average % 95
    Range % 90-100
    1:8000 Average % 88
    Range % 83-93
  • 回收率：
    The recovery of mouse FCN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 84 80-89
    EDTA plasma (n=4) 96 92-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    8000 2.495 2.569 2.532 2.385
    4000 2.014 2.071 2.043 1.896
    2000 1.622 1.584 1.603 1.456
    1000 1.178 1.113 1.146 0.999
    500 0.731 0.792 0.762 0.615
    250 0.456 0.428 0.442 0.295
    125 0.345 0.327 0.336 0.189
    0 0.147 0.146 0.147 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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Fcn2 Proteins

靶点详情

  • 功能：
    May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin.
  • 基因功能参考文献：
    1. this study shows that FCN B plays an important role in the development of collagen Ab-induced arthritis PMID: 28739878
    2. Ficolin-2 defends against virulent Mycobacteria tuberculosis infection in vivo, and its insufficiency is associated with infection in humans. PMID: 24040095
    3. Data show that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells. PMID: 23911405
    4. These results identify mouse ficolin-B as a functional member of the ficolin family activating complement via the lectin pathway. PMID: 22459270
    5. Results show that ficolin B in mouse bone marrow is an oligomeric protein. Ficolin B contained very low, but detectable, amounts of MASP-2 and sMAP and showed detectable C4-deposition activity on immobilized N-acetylglucosamine. PMID: 22523468
    6. Ficolin B exhibited a distinct ontogeny pattern that switched from embryonic liver to postnatal bone marrow and spleen. The cells that express ficolin B mRNA were identified as belonging to the myeloid cell lineage. PMID: 15950722
    7. Ficolin B showed multimeric structures and revealed binding to both N-acetylglucosamine and N-acetylgalactosamine. Ficolin B specifically recognized sialic acid residues. Ficolin B plays a distinct role through its unique carbohydrate-binding specificity. PMID: 16328467

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Ficolin lectin family
  • 数据库链接：

    KEGG: mmu:14134

    STRING: 10090.ENSMUSP00000028179

    UniGene: Mm.182935

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |