Mouse Fibulin-1(FBLN1) ELISA kit

Mouse Fibulin-1(FBLN1) ELISA kit

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中文名称：

小鼠腓骨蛋白-1(FBLN1)酶联免疫试剂盒
货号：

CSB-EL008452MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠腓骨蛋白-1(FBLN1)酶联免疫试剂盒（CSB-EL008452MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FBLN1含量。FBLN1（fibulin-1）是一种分泌性糖蛋白，主要存在于细胞外基质中，参与细胞粘附、迁移和信号传导。其研究机制涉及与层粘连蛋白和巢蛋白的结合，以及通过钙结合介导的血小板粘附。FBLN1的表达与多种疾病如动脉粥样硬化、癌症等密切相关。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于研究FBLN1在纤维化疾病模型中的动态变化、肿瘤微环境基质重塑机制、胚胎发育相关信号通路调控等科研领域，支持体外实验中对不同样本类型的FBLN1表达水平进行精确量化分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Fbln1Fibulin-1 ELISA kit; FIBL-1 ELISA kit; Basement-membrane protein 90 ELISA kit; BM-90 ELISA kit
缩写：

FBLN1
Uniprot No.：

Q08879
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	89-99
1:2000	Average %	95
	Range %	91-101
1:4000	Average %	94
	Range %	89-98
1:8000	Average %	98
	Range %	92-103

回收率：

The recovery of mouse FBLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-97
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.419	2.319	2.369	2.203
100	1.701	1.601	1.651	1.485
50	1.022	1.002	1.012	0.846
25	0.664	0.654	0.659	0.493
12.5	0.448	0.425	0.437	0.271
6.25	0.375	0.365	0.370	0.204
3.12	0.287	0.281	0.284	0.118
0	0.168	0.164	0.166

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.
基因功能参考文献：
1. Fbln1c plays a key role in regulating airway remodelling and inflammation in chronic asthma. PMID: 28862768
2. The FBLN1/FGF8 interaction may also be involved in the survival of neural crest cell population during development. PMID: 27402846
3. the findings indicate that Fbln1 normally suppresses proximal OFT EMT preventing proximal cushion hypercellularity and blood cell accumulation. PMID: 25575930
4. Fbln1 is a new positive modulator of the formation of membranous bone and endochondral bone in the skull PMID: 25201465
5. fibulin-1 regulates versican-dependent events in ventricular morphogenesis by promoting ADAMTS1 cleavage of versican leading to suppression of trabecular cardiomyocyte proliferation mediated by the ErbB2-Map kinase pathway. PMID: 22183742
6. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2 PMID: 11733994
7. structural and functional characterization of promoter PMID: 11829738
8. binding of HPV E6 protein to fibulin-1 PMID: 12200142
9. fibulin-1 is a new regulator of ADAMTS-1-mediated proteoglycan proteolysis and may play an important role in proteoglycan turnover in tissues where there is overlapping expression PMID: 16061471
10. A murine placental overgrowth phenotype is associated with elevated Fbln1 transcript levels. PMID: 16338003
11. Fbln1 is required for the directed migration and survival of cranial neueral crest cells contributing to the development of pharyngeal glands, craniofacial skeleton, cranial nerves, aortic arch arteries, cardiac outflow tract and cephalic blood vessels. PMID: 18538758
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亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Fibulin family
组织特异性：

Detected in most organs (brain, heart, lung, spleen, liver and kidney). Neurons are the predominant source of production in the brain. Not expressed significantly by astrocytes or microglia.
数据库链接：

KEGG: mmu:14114

STRING: 10090.ENSMUSP00000054583

UniGene: Mm.297992

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.