Mouse Fetuin A ELISA Kit
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中文名称：小鼠胎球蛋白A酶联免疫试剂盒
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货号：CSB-E14042m
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规格：96T/48T
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价格：￥3900/￥2500
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其他：
产品详情
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产品描述：CUSABIO小鼠胎球蛋白A（Fetuin-A）定量检测专用ELISA试剂盒（货号：CSB-E14042m），采用双抗体夹心法原理，可精准分析血清、血浆及组织匀浆样本中6.25-400 ng/mL浓度范围的靶蛋白。胎球蛋白A是由肝脏分泌的重要多功能糖蛋白，在钙磷代谢调节、胰岛素信号通路调控及炎症反应中发挥关键作用，其表达水平变化常与代谢综合征、动脉硬化等病理过程相关。试剂盒包含预包被96孔板、系列浓度标准品、生物素标记检测抗体及TMB显色系统，操作全程可在4小时内完成，灵敏度达6.25 ng/mL，适用于科研领域探究小鼠模型中肝脏功能异常、糖脂代谢紊乱、心血管疾病或骨代谢相关机制。其宽泛的样本兼容性可支持血清/血浆样本的体液分析，或通过组织匀浆检测特定器官的蛋白表达差异，为构建疾病模型、评估干预效果提供可靠数据支持。本产品严格验证批次稳定性，实验步骤标准化，配套提供详细操作指南及数据分析方案。
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别名：Ahsg ELISA kit; FetuaAlpha-2-HS-glycoprotein ELISA kit; Countertrypin ELISA kit; Fetuin-A ELISA kit
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缩写：AHSG
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Uniprot No.：
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma, tissue homogenates
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检测范围：6.25 ng/mL-400 ng/mL
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灵敏度：1.56 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Metabolism
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(ng/ml)
52.627
50.900
51.353
52.901
49.725
50.990
SD
0.041
0.035
0.037
0.054
0.049
0.047
CV(%)
5.216
4.563
4.793
6.844
6.499
6.120
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:10
Average %
95
Range %
87-103
1:20
Average %
95
Range %
84-105
1:40
Average %
90
Range %
83-103
1:80
Average %
93
Range %
82-107
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回收率：
The recovery of mouse fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
94
90-98
Heparin plasma (n=4)
91
85-96
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
400
2.657
2.704
2.681
2.547
200
1.905
2.002
1.954
1.820
100
1.259
1.279
1.269
1.135
50
0.714
0.758
0.736
0.602
25
0.426
0.442
0.434
0.300
12.5
0.361
0.348
0.355
0.221
6.25
0.257
0.244
0.251
0.117
0
0.134
0.133
0.134
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数据处理：
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货期：3-5 working days
产品评价
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靶点详情
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功能：Probably involved in differentiation.
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基因功能参考文献：
- Downregulation of adiponectin in inflamed adipocyte by fetuin-A through the mediation of Wnt3a and PPARgamma is a new report. PMID: 27720679
- FETUA expression and ER stress markers in the liver and serum of HFD mice were decreased by melatonin treatment. In conclusion, melatonin can improve hepatic insulin resistance and hepatic steatosis through reduction in ER stress and the resultant AHSG expression. PMID: 29607540
- Impairment of energy sensors, SIRT1 and AMPK, in lipid induced inflamed adipocyte is regulated by Fetuin A. PMID: 29030114
- The growth plate slippage occurred in 70% of Ahsg-/- mice of both sexes around three weeks postnatal. PMID: 29088242
- AHSG is produced in bone, mainly in osteocytes and also show for the first time that its production is modulated by FGF23. PMID: 26476373
- These findings establish urinary Fetuin-A as a sensitive biomarker of the progression of autosomal dominant polycystic kidney disease. PMID: 25888842
- Plasma levels of the calcification inhibitor fetuin-A were significantly decreased in CKD animals compared to controls. PMID: 24994603
- fetuin-A regulates endochondral ossification through mineralization inhibition and lipid (hormone) binding [review] PMID: 23277412
- Our results show that CSF fetuin-A is a biomarker of disease activity and natalizumab response in multiple sclerosis PMID: 23439582
- lipid-induced FetA from adipocytes is an efficient chemokine for macrophage migration and polarization PMID: 23943623
- FetA, via its terminal galactoside moiety, directly binds Leu100-Gly123 & Thr493-Thr516 in Tlr4, fulfilling the requirement of an endogenous ligand for TLR4 through which lipids induce insulin resistance. PMID: 22842477
- Our results show that fetuin-A has a developmental rather than a structural role in murine bone. PMID: 23091616
- Fetuin-A-containing calciprotein particles facilitate the clearance of mineral debris by macrophages via SR-A. PMID: 22753077
- ER stress induced by high glucose and palmitate increases the expression of fetuin-A and further contributes to the development of insulin resistance PMID: 22619360
- fetuin-A is protective against lethal systemic inflammation partly by inhibiting active HMGB1 release PMID: 21347455
- Fetuin-A deficiency is associated with soft tissue calcification in mice and humans. PMID: 21659653
- breast cancer but also of other tumors whose initiation and promotion are under the tight control of TGF-beta. PMID: 20847285
- Fetuin-A expressions were negatively correlated with the liver pathological scores and TNFa levels in fulminant hepatic failure. PMID: 18578995
- alpha 2-HS glycoprotein/fetuin, a transforming growth factor-beta/bone morphogenetic protein antagonist, regulates postnatal bone growth and remodeling PMID: 11901155
- Improved insulin sensitivity and resistance to weight gain in knockout mice PMID: 12145157
- role as a systemically acting inhibitor of ectopic calcification PMID: 12897203
- Regulation of BMP-induced ectopic bone formation by Ahsg. PMID: 15885488
- Onset of malaria infection is delayed in fetuin-A-deficient mice compared to that in wild-type C57BL/6 mice challenged with Plasmodium berghei sporozoites PMID: 16113307
- Fetuin-A knockout (fetuin-KO) mice spontaneously develop widespread soft tissue calcification, including significant myocardial calcification, whereas larger arteries are spared. PMID: 16177000
- The absence of fetuin may contribute to the improvement of insulin sensitivity associated with aging. PMID: 17011519
- Fetuin-A deficiency, chronic kidney disease, and high phosphate diet act synergistically in the pathogenesis of extraosseous calcification. PMID: 17389622
- fetuin recognized by anti-LY-R antibodies is an immunogenic form associated only with the tumorigenic LY-R cells and might be involved in tumor progression in this lymphoma. PMID: 19194829
- inhibits pathologic calcification in both the soft tissue and vasculature, even in the setting of atherosclerosis PMID: 19389852
- inhibition of ectopic calcification PMID: 12556469
- inhibition of ectopic calcification PMID: 9395485
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亚细胞定位：Secreted.
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蛋白家族：Fetuin family
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组织特异性：Liver is the major site of synthesis, but fetuin is also expressed in limb buds and other extrahepatic tissues during development.
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数据库链接：
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