Mouse Fatty acid synthase(FASN) ELISA kit
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中文名称：小鼠脂肪酸合成酶(FASN)酶联免疫试剂盒
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货号：CSB-EL008435MO
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：小鼠脂肪酸合成酶(FASN)酶联免疫试剂盒（CSB-EL008435MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FASN含量。FASN（脂肪酸合酶）是参与内源性脂肪酸合成的关键酶。其背景在于它在肿瘤等多种疾病中异常高表达。研究机制方面，主要聚焦于它促进肿瘤细胞增殖、存活、迁移等过程，抑制 FASN 活性有望为相关疾病治疗提供新策略。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品适用于基础科研中探究脂代谢调控机制、建立代谢性疾病动物模型或肿瘤模型后FASN的动态变化分析，以及药物干预实验中靶向FASN通路的疗效评估等研究场景，为揭示FASN在生理病理过程中的作用提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：FasnFatty acid synthase ELISA Kit; EC 2.3.1.85) [Includes: [Acyl-carrier-protein] S-acetyltransferase ELISA Kit; EC 2.3.1.38); [Acyl-carrier-protein] S-malonyltransferase ELISA Kit; EC 2.3.1.39); 3-oxoacyl-[acyl-carrier-protein] synthase ELISA Kit; EC 2.3.1.41); 3-oxoacyl-[acyl-carrier-protein] reductase ELISA Kit; EC 1.1.1.100); 3-hydroxyacyl-[acyl-carrier-protein] dehydratase ELISA Kit; EC 4.2.1.59); Enoyl-[acyl-carrier-protein] reductase ELISA Kit; EC 1.3.1.39); Oleoyl-[acyl-carrier-protein] hydrolase ELISA Kit; EC 3.1.2.14)] ELISA Kit
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缩写：
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Uniprot No.：
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma, tissue homogenates
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检测范围：3.12 pg/mL-200 pg/mL
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灵敏度：0.78 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Metabolism
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse FASN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:100
Average %
91
Range %
86-97
1:200
Average %
96
Range %
92-103
1:400
Average %
85
Range %
81-89
1:800
Average %
89
Range %
83-95
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回收率：
The recovery of mouse FASN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
91
87-95
EDTA plasma (n=4)
94
90-99
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
200
2.363
2.503
2.433
2.302
100
2.025
2.053
2.039
1.908
50
1.412
1.501
1.457
1.326
25
0.936
1.009
0.973
0.842
12.5
0.609
0.586
0.598
0.467
6.25
0.354
0.364
0.359
0.228
3.12
0.233
0.245
0.239
0.108
0
0.125
0.136
0.131
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数据处理：
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货期：3-5 working days
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靶点详情
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功能：Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
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基因功能参考文献：
- an unexpected link between FASN and cholesterol synthesis that appears to be required for TLR signal transduction and proinflammatory macrophage activation. PMID: 29463677
- FASN regulates the pathogenicity of Th17 cells. PMID: 28848043
- genetic deletion of FASN totally suppresses hepatocarcinogenesis driven by AKT and AKT/c-Met protooncogenes in mice. PMID: 28118080
- Data show that nuclear factor Y (NF-Y) binds to the inverted CCAAT element (ICE) in the Fatty acid synthase (Fasn) promoter specifically in refeeding states. PMID: 28281280
- study demonstrates that co-activation of AKT and c-Met induces hepatocellular carcinoma development that depends on the mTORC1/FASN pathway. PMID: 26857837
- Inhibition of G-protein-coupled Receptor Kinase 2 Prevents the Dysfunctional Cardiac Substrate Metabolism in Fatty Acid Synthase Transgenic Mice. PMID: 26670611
- These findings suggest that activation of TSHR directly inhibits FASN expression in mature adipocytes, possibly mediated by PKA and ERK PMID: 25655684
- UCP2-induced fatty acid synthase promotes NLRP3 inflammasome activation during sepsis PMID: 25574840
- FASN plays an important role in exercise-mediated cognitive enhancement, which might be associated to its role in modulating exercise-induced stimulation of neurogenesis. PMID: 24223732
- FASN is essential for the development, functional competence, and maintenance of the lactating mammary gland. PMID: 24668799
- Gamma-tocotrienol showed attenuation of triglyceride through effect on fatty acid synthase, sterol regulatory element-binding transcription factor 1, stearoyl CoA desaturase 1, and carnitine palmitoyl transferase 1A gene expression in Hepa 1-6 cells. PMID: 23727646
- Upregulation of host hepatic fatty acid synthase correlates with hepatitis B virus replication. PMID: 23675653
- Modulation of fatty acid synthase degradation by concerted action of p38 MAP kinase, E3 ligase COP1, and SH2-tyrosine phosphatase Shp2. PMID: 23269672
- fatty acid synthase (Fasn), the key enzyme of de novo lipogenesis, is highly active in adult neural stem and progenitor cells and conditional deletion of Fasn in mouse NSPCs impairs adult neurogenesis PMID: 23201681
- Kr-pok (kidney cancer-related POZ domain and Kruppel-like protein) activates transcription of the FASN gene. PMID: 22331133
- FAS deficiency blocked the generation of palmitoylated Mucin 2, which must be S-palmitoylated at its N terminus for proper secretion and function. PMID: 22341463
- Fatty acid synthase modulates homeostatic responses to myocardial stress PMID: 21757749
- These findings pertinent to the etiology of tumor metabolism also underscore the key role of the Sonic hedgehog-->E2F1-->FASN axis in regulating de novo lipid synthesis in cancers. PMID: 20890301
- Fatty acid synthase may serve as a new diagnostic marker or therapeutic target for the progression of nonalcoholic fatty liver disease PMID: 20606731
- FAS, which is induced by LXRalpha, may generate regulatory lipids that cause feedback inhibition of LXRalpha in macrophages. PMID: 20479009
- FAS is present and active in neurons in the hypothalamus PMID: 12376313
- role in fatty acid synthesis PMID: 12738878
- fatty acid synthase gene is regulated by SREBP PMID: 12897158
- Together, these results suggest that FAS generated signals may be essential to support preadipocyte differentiation. PMID: 15707987
- Insulin acutely reduces hepatic FAS activity by inducing phosphorylation of the carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) and its interaction with FAS. PMID: 16054098
- Inhibition of hypothalamic fatty acid synthase triggers rapid activation of fatty acid oxidation in skeletal muscle. PMID: 16203972
- AMP-activated protein kinase-induced Fatty acid synthase phosphorylation inhibits lipogenesis. PMID: 17635921
- 2 diametrically opposed proteins, fatty acid synthase (induced by feeding) and PPARalpha (induced by starvation), unexpectedly form an integrative sensory module in the central nervous system to orchestrate energy balance PMID: 17694178
- PPARalpha is required to mediate the response of Scd-1 and Fas gene expression to insulin and that this is normally achieved directly by activation of LXRalpha. PMID: 18079124
- Proto-oncogene FBI-1 (Pokemon) and SREBP-1 synergistically activate transcription of fatty-acid synthase gene PMID: 18682402
- Report effect of centrally administered C75, a fatty acid synthase inhibitor, on gastric emptying and gastrointestinal transit in mice. PMID: 18706411
- FASN activity is essential for B16-F10 melanoma cell proliferation and survival while its inactivation by Orlistat significantly reduces their metastatic spread PMID: 18770866
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亚细胞定位：Cytoplasm. Melanosome.
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