Mouse Epidermal growth factor-like protein 6(EGFL6) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠表皮生长因子样蛋白6(EGFL6)酶联免疫试剂盒（CSB-EL007475MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EGFL6含量。EGFL6是一种重要靶点。它在细胞增殖、迁移等生理过程中发挥作用。研究发现其异常表达与多种疾病相关，如肿瘤的发生发展。科研人员正深入探究其作用机制，期望通过靶向EGFL6开发新的诊断方法和治疗策略。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，科研人员可通过探究EGFL6在小鼠模型中的功能特性，例如在肿瘤发生、血管异常增生或创伤修复等研究领域，定量分析不同实验条件下EGFL6的动态变化，为疾病机制研究或药物干预效果评估提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Egfl6 ELISA Kit; Maeg ELISA Kit; Epidermal growth factor-like protein 6 ELISA Kit; EGF-like protein 6 ELISA Kit; MAM and EGF domains-containing gene protein ELISA Kit; Protein W80 ELISA Kit
缩写：

EGFL6
Uniprot No.：

Q9JJZ5
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

93.75 pg/mL-6000 pg/mL
灵敏度：

23.43 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse EGFL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	98
	Range %	94-101
1:4	Average %	102
	Range %	94-108
1:8	Average %	94
	Range %	87-97

回收率：

The recovery of mouse EGFL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-103
EDTA plasma (n=4)	93	89-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly.
基因功能参考文献：
1. EGLF6 mediates a paracrine mechanism of cross-talk between vascular endothelial cells and osteoblasts PMID: 21531721
2. MAEG may play a role as a mediator regulating epithelial-mesenchymal interaction through binding to RGD-binding integrins including alpha8beta1 during hair follicle development. PMID: 15572035
亚细胞定位：

Secreted, extracellular space, extracellular matrix, basement membrane.
蛋白家族：

Nephronectin family
组织特异性：

Expressed at basement membrane of pelage follicles (at protein level).
数据库链接：

KEGG: mmu:54156

UniGene: Mm.37707