Mouse Eotaxin 3 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠嗜酸粒细胞趋化蛋白Eotaxin 3(Eotaxin 3)酶联免疫试剂盒（CSB-E15062m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的Eotaxin 3含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于研究炎症性疾病模型中嗜酸性粒细胞募集机制、评估实验性抗过敏药物对趋化因子表达的调控作用，或解析免疫细胞与微环境相互作用的分子基础，为呼吸道疾病、皮肤过敏等病理机制研究提供可靠工具。试剂盒操作流程标准化，适用于体外实验环境下多种生物样本的平行检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

Eotaxin 3
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Eotaxin 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-93
1:2	Average %	96
	Range %	92-100
1:4	Average %	103
	Range %	101-105
1:8	Average %	89
	Range %	84-94

回收率：

The recovery of mouse Eotaxin 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-94
EDTA plasma (n=4)	89	85-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.