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  4. Mouse Endosialin(CD248) ELISA kit

Mouse Endosialin(CD248) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠内皮唾酸蛋白(CD248)酶联免疫试剂盒
  • 货号：
    CSB-EL004905MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠内皮唾酸蛋白(CD248)酶联免疫试剂盒（CSB-EL004905MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD248含量。CD248，又称内皮唾液酸蛋白，是一种在肿瘤相关成纤维细胞和周细胞中表达的肿瘤标志物。其研究机制涉及调节Wnt信号通路，促进血管生成和肿瘤生长，在多种肿瘤中发挥重要作用。试剂盒检测范围为7.81 pg/mL-500 pg/mL，该产品适用于科研领域对血管生成机制、肿瘤微环境调控或纤维化疾病模型的探索性研究，为体外定量分析CD248在生理或病理状态下的动态变化提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cd248 ELISA Kit; Tem1 ELISA Kit; Endosialin ELISA Kit; Tumor endothelial marker 1 ELISA Kit; CD antigen CD248 ELISA Kit
  • 缩写：
    CD248
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.81 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD248 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:100 Average % 95
    Range % 90-99
    1:200 Average % 102
    Range % 96-107
    1:400 Average % 93
    Range % 89-98
    1:800 Average % 86
    Range % 80-92
  • 回收率：
    The recovery of mouse CD248 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 93-102
    EDTA plasma (n=4) 98 94-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    500 2.643 2.781 2.712 2.625
    250 1.765 1.822 1.794 1.707
    125 1.121 1.211 1.166 1.079
    62.5 0.721 0.710 0.716 0.629
    31.25 0.433 0.448 0.441 0.354
    15.62 0.276 0.282 0.279 0.192
    7.81 0.182 0.188 0.185 0.098
    0 0.086 0.088 0.087 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Cd248 Proteins

靶点详情

  • 功能：
    May play a role in angiogenesis or vascular function.
  • 基因功能参考文献：
    1. the data demonstrate a critical role for endosialin-expressing primary tumor pericytes in mediating metastatic dissemination and identify endosialin as a promising therapeutic target in breast cancer PMID: 27635044
    2. Our data show that deletion of CD248 reduces susceptibility to liver fibrosis via an effect on PDGF signaling. PMID: 26078290
    3. Endosialin is a potential regulator of phenotypic remodeling of vascular smooth muscle cells contributing to atherosclerosis. PMID: 28126825
    4. suggest that CD248 stromal cells have a pathogenic role in renal fibrosis PMID: 26633297
    5. We conclude that CD248 is required for PDGFRbeta-dependant capillary sprouting but not splitting angiogenesis, and identify a new role for CD248 expressed on pericytes in the early stages of physiological angiogenesis during muscle remodelling. PMID: 25243742
    6. Tem1-TT vaccination elicited CD8+ and/or CD4+ T cell responses against immunodominant TEM1 protein sequences. Prophylactic immunization of animals with Tem1-TT prevented or delayed tumor formation in several tumor models. PMID: 24642465
    7. Expression of CD248 was seen in osteoblasts, but not osteoclasts. CD248(-/-) mouse tibiae had higher bone mass and superior mechanical properties compared to WT mice. Primary osteoblasts from CD248(-/-) mice induced increased bone mineralization PMID: 22674221
    8. The multiple pathways regulated by the cytoplasmic domain of CD248 highlight its potential as a therapeutic target to treat cancer. PMID: 21549007
    9. our observations suggest that Tem1 is expressed throughout embryonic and adult development in several types of mesenchymal cells closely related to blood vessels. PMID: 21402174
    10. required for efficient lymph node expansion PMID: 20432232
    11. Tem1 have significant implications for the mechanisms underlying tumor invasiveness and for models that evaluate this process PMID: 16492758
    12. In the embryo when analysed by confocal microscopy endosialin on vessels does not colocalise with endothelial cells expressing CD31, rather, endosialin is restricted to closely associated perivascular cells that also express the pericyte marker NG2. PMID: 16965941
    13. CD248 expression helps define a subset of lymphoid tissue stromal cells which play a role in remodelling during tissue development, infection and repair PMID: 17628549

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed in cell lines derived from endothelial cells, embryonic fibroblasts and preadipocytes.
  • 数据库链接：

    KEGG: mmu:70445

    STRING: 10090.ENSMUSP00000070847

    UniGene: Mm.29597

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |