Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit

Mouse E3 ubiquitin-protein ligase SMURF1(SMURF1) ELISA kit

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中文名称：

小鼠E3泛素连接酶SMURF1(SMURF1)酶联免疫试剂盒
货号：

CSB-EL021860MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠E3泛素连接酶SMURF1（SMURF1）定量检测专用ELISA试剂盒（货号：CSB-EL021860MO），采用双抗体夹心法原理，可精准检测血清、血浆、组织匀浆及细胞裂解液等多种样本中SMURF1的浓度，灵敏度达7.8 pg/mL，检测范围为31.25-2000 pg/mL。SMURF1作为HECT型E3泛素连接酶家族成员，通过调控TGF-β/BMP信号通路参与细胞增殖、分化及凋亡过程，在骨组织发育、肿瘤发生等生理病理过程中发挥重要作用。本试剂盒可在3.5小时内完成检测流程，仅需50μL样本即可实现高特异性分析，配套提供标准品、检测抗体及预包被酶标板，适用于体外培养细胞或动物模型中SMURF1蛋白表达水平的动态监测，为研究泛素化修饰机制、信号转导调控及疾病相关分子网络提供可靠工具，特别适合骨代谢研究、肿瘤微环境分析及药物干预效果评估等科研场景。
别名：

Smurf1 ELISA Kit; E3 ubiquitin-protein ligase SMURF1 ELISA Kit; EC 2.3.2.26 ELISA Kit; HECT-type E3 ubiquitin transferase SMURF1 ELISA Kit; SMAD ubiquitination regulatory factor 1 ELISA Kit; SMAD-specific E3 ubiquitin-protein ligase 1 ELISA Kit
缩写：

SMURF1
Uniprot No.：

Q9CUN6
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SMURF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	92
	Range %	88-95
1:4	Average %	86
	Range %	80-91
1:8	Average %	89
	Range %	83-98

回收率：

The recovery of mouse SMURF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	101	94-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.804	2.600	2.702	2.535
1000	2.403	2.371	2.387	2.220
500	1.747	1.850	1.799	1.632
250	0.972	0.975	0.974	0.807
125	0.594	0.581	0.588	0.421
62.5	0.370	0.347	0.359	0.192
31.25	0.256	0.245	0.251	0.084
0	0.170	0.163	0.167

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
基因功能参考文献：
1. Inhibition of osteoblastic Smurf1 promotes bone formation in mouse models of distinctive age-related osteoporosis PMID: 30143635
2. Smurf1 expression in osteoblast differentiation is regulated by CRTC2. PMID: 30449452
3. Smurf1 interacts with and targets Securin, an inhibitor of sister-chromatid separation, for poly-ubiquitination and proteasomal degradation. PMID: 28658604
4. Six2 mediates the protective effects of GDNF on damaged DA neurons by regulating Smurf1 expression. PMID: 27148690
5. TGF-beta inhibited osteoblastic differentiation by inducing the MAPK-ERK pathway which upregulated the expression of ubiquitin ligase SMURF1 and resulted in reduced presence of osteogenic proteins. PMID: 28322449
6. demonstration that Smurf1 acts as a brake for integrin activation by controlling Kindlin-2 protein levels, a new mechanism that permits precise modulation of integrin-mediated cellular functions PMID: 28408404
7. miR-140-5p and SMURF1 are key regulators of disease pathology in pulmonary arterial hypertension PMID: 27214554
8. Smurf1 is required for selective autophagy of Mycobacterium tuberculosis and host defense against tuberculosis infection. PMID: 28017659
9. Smurf1 regulates glucose metabolism and inhibits osteoblast differentiation. Smurf1 function requires the presence of serine 148 (S148) in Smurf1. PMID: 27052174
10. Smurf1 mediates NGF signaling and ubiquitinates RhoA in growth cones, regulating the need for local translation and degradation. PMID: 25901863
11. Smurf-mediated endocytosis of Patched1 is required in sonic hedgehog signal reception PMID: 24925320
12. Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cells. PMID: 24288199
13. Smurf1-mediated Lys29-linked nonproteolytic polyubiquitination of axin1 negatively regulates Wnt/beta-catenin signaling. PMID: 23959799
14. These results suggest that the JNK/AP-1 and ERK/Runx2 signaling pathways mediate TNF-alpha-dependent Smurf1 transcription. PMID: 23042144
15. Smurf1 is a negative feedback regulator for IFN-gamma signaling by targeting STAT1 for ubiquitination and proteasomal degradation. PMID: 22474288
16. studies uncover a cell-cycle-independent function of Cdh1, establishing Cdh1 as an upstream component that governs Smurf1 activity PMID: 22152476
17. ER stress induces Smurf1 degradation and WFS1 up-regulation PMID: 21454619
18. Smurf1 negatively regulates mesenchymal stem cell proliferation and differentiation by controlling JunB turnover through an ubiquitin-proteasome pathway. PMID: 20200942
19. induction could trigger downregulation of bone morphogenetic protein signaling and cause vascular cell proliferation and remodeling in pulmonary arterial hypertension pulmonary arterial hypertension PMID: 20558834
20. Smurf1 appears to be an important regulatory factor in osteoblast differentiation and a potential molecular target for identification of bone anabolic agents PMID: 12738770
21. Smurf1 is required for the myogenic differentiation of C2C12 cells and plays an important regulatory role in the BMP-2-mediated osteoblast conversion PMID: 12871975
22. Smurf1 plays a specific role in osteoblast differentiation and bone formation in vivo PMID: 14701828
23. overexpression of Smurf1 specifically promotes Smad1 and Smad5 ubiquitination and degradation in embryonic lung epithelium PMID: 14711801
24. Mating Smad6 and Smurf1 transgenic mice produced double-transgenic pups with more delayed endochondral ossification than Smad6 transgenic mice, suggesting that Smurf1 supports Smad6 function in vivo. PMID: 15123739
25. Smurf1 negatively regulates osteoblast activity and response to BMP through controlling MEKK2 degradation. PMID: 15820682
26. in chronic inflammatory disorders where TNF is increased, TNF induces the expression of ubiquitin ligase Smurf1 and promotes ubiquitination and proteasomal degradation of Smad1 and Runx2, leading to systemic bone loss PMID: 18567580
27. the WW domains linker is important in complex assembly and in regulating activity of HECT-type E3s and CKIP-1 functions as the first auxiliary factor to enhance the activation of Smurf1. PMID: 18641638
28. Mice mutant for Smurf1 and Smurf2 display planar cell polarity defects in the cochlea and convergence and extension movements defects that include a failure to close the neural tube. PMID: 19379695
29. Initial identification of Smurf1 as an ubiquitin ligase that inhibits BMP signaling by targeting Smad1/5 for proteasomal destruction in cultured cells and Xenopus embryos. PMID: 10458166
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亚细胞定位：

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
数据库链接：

KEGG: mmu:75788

STRING: 10090.ENSMUSP00000082827

UniGene: Mm.27735

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.