Mouse Desmosine ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠锁链素（Desmosine）酶联免疫检测试剂盒（货号：CSB-E14196m），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中锁链素的含量，检测灵敏度范围为3.12-200 pg/mL。锁链素是弹性蛋白特异性交联氨基酸，广泛存在于肺、血管等弹性纤维中，其释放水平可反映组织弹性蛋白的降解程度，常用于研究肺部纤维化、慢性阻塞性肺病（COPD）及皮肤老化等相关机制。试剂盒包含预包被捕获抗体的96孔板、锁链素标准品、生物素标记检测抗体及显色底物，操作流程包括样本孵育、洗涤、信号放大与读数，可在4.5小时内完成检测。该试剂盒适用于科研场景下的小鼠模型研究，如评估疾病进程中弹性蛋白代谢异常、验证药物对组织修复的影响或探索衰老相关分子机制。实验需准备酶标仪（450 nm波长），样本需离心去除颗粒物，组织匀浆建议按1:5比例稀释后检测。本试剂盒具有高特异性和稳定性，支持低至50 μL样本量，为弹性蛋白研究提供可靠工具。
缩写：

Desmosine
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Desmosine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	85
	Range %	81-92
1:20	Average %	96
	Range %	91-103
1:40	Average %	103
	Range %	97-107
1:80	Average %	98
	Range %	92-102

回收率：

The recovery of mouse Desmosine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-96
EDTA plasma (n=4)	104	96-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.