Mouse D-Lactate Dehydrogenase,D-LDH ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠D-乳酸脱氢酶(D-LDH)酶联免疫试剂盒（CSB-E11723m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的LDHD含量。试剂盒检测范围为4.69 mU/mL-300 mU/mL，适用于科研领域中对小鼠模型中D-LDH功能的研究，例如肠道缺血再灌注损伤机制探索、糖尿病相关代谢异常评估或细胞能量代谢实验等场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Ldhd ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit; mitochondrial ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; EC 1.1.2.4 ELISA Kit
缩写：

LDHD
Uniprot No.：

Q7TNG8
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

4.69 mU/mL-300 mU/mL
灵敏度：

1.17 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	93
	Range %	90-96
1:4	Average %	87
	Range %	83-90
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of mouse D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	104	101-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in D-lactate, but not L-lactate catabolic process.
基因功能参考文献：
1. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
亚细胞定位：

Mitochondrion.
蛋白家族：

FAD-binding oxidoreductase/transferase type 4 family
组织特异性：

Readily detected in liver and kidney, with a weaker signal observed in heart, skeletal muscle, stomach, brain, and lung.
数据库链接：

KEGG: mmu:52815

STRING: 10090.ENSMUSP00000068086

UniGene: Mm.271578