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  4. Mouse Cytochrome-C,Cyt-C ELISA Kit

Mouse Cytochrome-C,Cyt-C ELISA Kit

文献引用 (5) 说明书下载
  • 中文名称：
    小鼠细胞色素C(Cyt-C)酶联免疫试剂盒
  • 货号：
    CSB-E08532m
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠细胞色素C(Cyt-C)酶联免疫试剂盒（CSB-E08532m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYCS含量。CYCS即细胞色素 c，是线粒体呼吸链的重要组成部分。它在细胞呼吸和能量代谢中起关键作用。研究发现，CYCS还参与细胞凋亡机制，当细胞受到凋亡信号刺激时，它会从线粒体释放到胞质中，激活凋亡相关酶，引发细胞程序性死亡。试剂盒检测范围为34.3 pg/mL-2200 pg/mL，适用于科研场景下细胞凋亡机制及线粒体功能相关研究；可为神经退行性疾病模型、心肌缺血再灌注损伤、肿瘤治疗响应等基础研究提供关键数据；适用于实验室规模的高通量筛选或小样本量研究，尤其在药物干预后线粒体功能评估、基因编辑模型验证等场景中具有重要应用价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cycs ELISA Kit; Cytochrome c ELISA Kit; somatic ELISA Kit
  • 缩写：
    CYCS
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    34.3 pg/mL-2200 pg/mL
  • 灵敏度：
    8.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Cyt-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 88-97
    1:2 Average % 84
    Range % 80-88
    1:4 Average % 90
    Range % 85-94
    1:8 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of mouse Cyt-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 95-105
    EDTA plasma (n=4) 87 83-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    2200 2.352 2.512 2.432 2.240
    1100 1.615 1.578 1.597 1.405
    550 1.073 1.023 1.048 0.856
    275 0.639 0.681 0.660 0.468
    137.5 0.461 0.489 0.475 0.283
    68.75 0.387 0.374 0.381 0.189
    34.3 0.318 0.305 0.312 0.120
    0 0.194 0.189 0.192 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Cycs Proteins

靶点详情

  • 功能：
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 基因功能参考文献：
    1. the amount of cytochrome c in lymphomyeloid cells does not affect their development and normal functioning PMID: 25716737
    2. Data suggest that peroxidase activation of cytochrome c may induce apoptosis and contribute to anti-cancer properties of alpha-tocopherol succinate. PMID: 25278024
    3. Data indicate that mitochondrial alpha 7 nicotinic acetylcholine receptors (alpha7 nAChRs) regulate cytochrome c (cyt c) release by engaging intramitochondrial protein kinases. PMID: 24412630
    4. proposed that mutation of residue 41, and interaction with cardiolipin, increase peroxidase activity by altering the 40-57 Omega loop and its hydrogen bond network with the propionate of haem ring A; these changes enhance access of hydrogen peroxide and substrate to the haem PMID: 24329121
    5. Data indicate that Y48H missense mutation of cytochrome c (CYCS) gene is associated with respiratory reduction and increased apoptosis in fibroblast. PMID: 24326104
    6. Translocation of a Bak C-terminus mutant from cytosol to mitochondria to mediate cytochrome C release: implications for Bak and Bax apoptotic function. PMID: 22442658
    7. mitochondrial import and direct electron transfer from cytochrome c to Rac1 modulates mitochondrial H(2)O(2) production in alveolar macrophages pulmonary fibrosis. PMID: 22157762
    8. The course of etoposide-induced apoptosis from damage to DNA and p53 activation to mitochondrial release of cytochrome c. PMID: 11864976
    9. results suggest that activation of mitochondrial ATP-dependent potassium channels protects neurons against apoptosis by preventing cytochrome c release from the mitochondria PMID: 11919514
    10. Both neurons and cancer cells strictly inhibit cytochrome c-mediated apoptosis by a mechanism dependent on glucose metabolism. PMID: 19029908

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  • 亚细胞定位：
    Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
  • 蛋白家族：
    Cytochrome c family
  • 组织特异性：
    Found in embryos and in adult liver and heart.
  • 数据库链接：

    KEGG: mmu:13063

    STRING: 10090.ENSMUSP00000124523

    UniGene: Mm.35389

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |