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  4. Mouse Cystatin-F(CST7) ELISA kit

Mouse Cystatin-F(CST7) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    小鼠胱抑素F(CST7)酶联免疫试剂盒
  • 货号：
    CSB-EL006095MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    小鼠胱抑素F(CST7)酶联免疫试剂盒（CSB-EL006095MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CST7含量。小鼠胱抑素F（CST7）是一种具有半胱氨酸型内肽酶抑制活性的蛋白质，参与肽酶活性的负调控，在抑制半胱氨酸型内肽酶活性、负调控小胶质细胞激活和正调控髓鞘化等过程中发挥作用，其表达与神经炎症和神经病理性疼痛有关。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于神经科学、免疫学等领域的基础研究，可应用于实验动物模型（如神经损伤、炎症性疾病模型）中胱抑素F的动态监测，为探究其与疾病发生发展的关联提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cst7Cystatin-F ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; CMAP ELISA Kit; Leukocystatin ELISA Kit
  • 缩写：
    CST7
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 89
    Range % 84-93
    1:2 Average % 100
    Range % 95-105
    1:4 Average % 87
    Range % 83-90
    1:8 Average % 104
    Range % 100-107
  • 回收率：
    The recovery of mouse CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 86 83-89
    EDTA plasma (n=4) 95 91-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.547 2.601 2.574 2.452
    5 2.120 2.032 2.076 1.954
    2.5 1.394 1.402 1.398 1.276
    1.25 0.820 0.805 0.813 0.691
    0.625 0.517 0.531 0.524 0.402
    0.312 0.325 0.326 0.326 0.204
    0.156 0.239 0.242 0.241 0.119
    0 0.121 0.123 0.122 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

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Cst7 Proteins

靶点详情

  • 功能：
    Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
  • 基因功能参考文献：
    1. High CST7 expression is associated with Alzheimer's disease. PMID: 28904096
    2. Our results validate cystatin F as a useful biomarker of early pathogenesis in experimental models of prion disease, and point to unexpected species-specific differences in the transcriptional responses to prion infections. PMID: 28178353
    3. During the active remyelinating phase, both CysF knockdown (CysFKD) and microglial-selective CatC overexpression (CatCOE) showed a worsening of the demyelination in Plp(4e/-) transgenic mice. Conversely, during the chronic demyelinating phase, CatC knockdown (CatCKD) ameliorated the demyelination. Our results suggest that the balance between CatC and CysF expression controls the demyelination and remyelination process. PMID: 28251676
    4. The cystatin F expression in the activated microglia is closely associated with the effect of the A2A receptors, which may be related to the neuroinflammatory responses occurring during the pathological process. PMID: 23285090
    5. an inhibitory activity other than cystatin F quenches CatL activity in activated macrophages PMID: 21956111
    6. Expression of cystatin F indicates ongoing demyelination and remyelination; absence of cystatin F expression indicates cessation of remyelination in the demyelinating area. PMID: 21344476

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 数据库链接：

    KEGG: mmu:13011

    STRING: 10090.ENSMUSP00000086606

    UniGene: Mm.12965

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |