Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

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中文名称：

小鼠促肾上腺皮质激素释放因子结合蛋白(CRHBP)酶联免疫试剂盒
货号：

CSB-EL005964MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠促肾上腺皮质激素释放因子结合蛋白(CRHBP)酶联免疫试剂盒（CSB-EL005964MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CRHBP含量。CRHBP即促肾上腺皮质激素释放激素结合蛋白，它在应激反应调节等方面有重要作用。其研究机制主要围绕它与促肾上腺皮质激素释放激素（CRH）的结合展开，通过结合CRH影响其生物活性，进而调控下丘脑-垂体-肾上腺轴（HPA轴）的功能。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，参与应激反应、能量代谢及神经内分泌功能的动态平衡研究；探究应激相关疾病模型中CRHBP的动态变化、评估HPA轴功能异常相关机制，或用于神经内分泌调控的基础研究；助力于神经生物学、内分泌学及行为医学等领域的实验研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CrhbpCorticotropin-releasing factor-binding protein ELISA kit; CRF-BP ELISA kit; CRF-binding protein ELISA kit; Corticotropin-releasing hormone-binding protein ELISA kit; CRH-BP ELISA kit
缩写：

CRHBP
Uniprot No.：

Q60571
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CRHBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-97
1:2	Average %	96
	Range %	91-103
1:4	Average %	97
	Range %	94-100
1:8	Average %	93
	Range %	88-98

回收率：

The recovery of mouse CRHBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-100
EDTA plasma (n=4)	91	84-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.582	2.776	2.679	2.501
500	2.193	2.223	2.208	2.030
250	1.446	1.465	1.456	1.278
125	0.821	0.865	0.843	0.665
62.5	0.565	0.612	0.589	0.411
31.25	0.319	0.295	0.307	0.129
15.6	0.256	0.246	0.251	0.073
0	0.178	0.177	0.178

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.
基因功能参考文献：
1. Repeated cycles of binge drinking alter CRF-BP mRNA expression in the medial prefrontal cortex. PMID: 27374820
2. Ventral tegmental area CRH-BP is involved in the initial stages of escalated ethanol drinking. PMID: 26247973
3. These studies demonstrate a role for the pituitary CRH-BP in attenuating the HPA response to stress in female mice. PMID: 25731977
4. estrogen-regulated expression of corticotropin-releasing hormone-binding protein in lactotropes in female mice suggests that the pituitary is an important site for interactions between the hypothalamic-pituitary-adrenal axis and other endocrine systems PMID: 12446601
5. Evidence is presented suggesting that the CRH-BP may be required for corticotropin-releasing factor-induced potentiation of NMDA receptors in dopaminergic neurons of the ventral tegmental area. PMID: 12895416
6. GnRH induces a 3.7-fold increase in CRH-BP mRNA levels via multiple intracellular signaling pathways and a multipartite GnRH response element. PMID: 15976007
7. analysis of regulation and function of CRH-BP [review] PMID: 16368564
8. Deletion of corticotropin-releasing factor binding protein selectively impairs maternal, but not intermale aggression. PMID: 18929624
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亚细胞定位：

Secreted.
蛋白家族：

CRF-binding protein family
数据库链接：

KEGG: mmu:12919

STRING: 10090.ENSMUSP00000042578

UniGene: Mm.316614

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.