Mouse Carnitine O-palmitoyltransferase 1, liver isoform(CPT1A) ELISA kit

Mouse Carnitine O-palmitoyltransferase 1, liver isoform(CPT1A) ELISA kit

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中文名称：

小鼠肉毒碱-棕榈酰基转移酶1，肝脏亚型(CPT1A)酶联免疫试剂盒
货号：

CSB-EL005922MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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小鼠肉毒碱-棕榈酰基转移酶1，肝脏亚型(CPT1A)酶联免疫试剂盒（CSB-EL005922MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的CPT1A含量。CPT1A是一种重要的靶点。它在脂肪酸代谢中扮演关键角色，催化长链脂肪酸转运入线粒体进行β-氧化。对其研究发现，异常的CPT1A活性与多种疾病如代谢综合征、癌症等相关，探索其作用机制有望为相关疾病治疗提供新方向。试剂盒检测范围为6.25 pg/mL-400 pg/mL，该产品可应用于基础医学研究中肝脏代谢功能评估、肥胖或糖尿病动物模型能量代谢通路分析、药物干预对脂肪酸氧化影响的体外实验等领域，为探索脂质代谢调控机制及相关疾病发病机理提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Cpt1a; Cpt-1; Cpt1; Carnitine O-palmitoyltransferase 1, liver isoform; CPT1-L; Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
缩写：

CPT1A
Uniprot No.：

P97742
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell lysates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CPT1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-100
1:2	Average %	96
	Range %	87-102
1:4	Average %	90
	Range %	85-99
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of mouse CPT1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	86-104
EDTA plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.006	2.034	2.020	1.843
200	1.372	1.385	1.379	1.202
100	0.735	0.765	0.750	0.573
50	0.431	0.442	0.437	0.260
25	0.286	0.291	0.289	0.112
12.5	0.237	0.248	0.243	0.066
6.25	0.215	0.223	0.219	0.042
0	0.174	0.179	0.177

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in hepatic triglyceride metabolism.
基因功能参考文献：
1. Alogliptin increased hepatic mRNA expression levels associated with fatty acid oxidation. In addition, the results of the present study suggested that ALO promotes CPT1a expression via Thr172 phosphorylation of AMPKalpha. PMID: 29512720
2. Furthermore, given the already low abundance of Cpt1b in white adipose tissue, it is unlikely that decreases in its expression can quantitatively decrease whole body energy expenditure enough to contribute to an obese phenotype. PMID: 28330968
3. beneficial effects of muscle CPT1mt expression suggest that a direct modulation of the malonyl-CoA/CPT1 partnership in skeletal muscle could represent a potential strategy to prevent obesity-induced insulin resistance PMID: 27507552
4. The data suggest that AMPK is not required for the regulation of the intermediate filament interaction with CPT-I during exercise. PMID: 27941154
5. These results suggest that n-3 polyunsaturated fatty acids could improve lipid oxidation-related enzymes in liver subjected to hemorrhagic shock/resuscitation. PMID: 27110821
6. these results demonstrated that L-carnitine ameliorated liver inflammation and serum pro-inflammatory markers in cancer cachexia through regulating CPT I-dependent PPARg signaling PMID: 26647854
7. Data show that peroxisome-proliferator-activated receptor beta/delta (PPARbeta/delta) activation restored the lipid-induced endothelial dysfunction by up-regulation of carnitine palmitoyltransferase)-1 (CPT-1). PMID: 26253087
8. Our results indicated that exercise-induced CPT1b expression was at least in part mediated by HDAC5/MEF2A interaction. PMID: 25213552
9. Data indicate that muscle mutated carnitine palmitoyltransferase 1 (CPT1mt) expression enhanced mitochondrial fatty acid oxidation (mFAO) capacity. PMID: 25713059
10. Potential epigenetic mechanisms underlying transcriptional regulation of Cpt1alpha in hepatic steatosis occur in response to binge ethanol administration. PMID: 23905631
11. an environment-dependent structural switch underlies the regulation of carnitine palmitoyltransferase 1A PMID: 21990363
12. miR-370 acting via miR-122 may have a causative role in the accumulation of hepatic triglycerides by modulating initially the expression of SREBP-1c, DGAT2, and Cpt1alpha. PMID: 20124555
13. Results show that CREB and HNF4alpha bind the CPT promoter, that PGC-1 is involved in liver carnitine palmitoyltransferase I (CPT) gene activation by cylic AMP, and that PGC-1 acts to coordinate the process of metabolic adaptation in the liver. PMID: 12107181
14. role of thyroid hormone response unit formed between the promoter and first intron in mediating liver-specific induction by thyroid hormone PMID: 12493735
15. Malonyl-CoA/CPTI interaction is a component of a metabolic signaling network that controls insulin secretion. PMID: 15677504
16. modulation of CPT1A expression by PI3K-dependent signaling is the major mechanism by which cells suppress beta-oxidation during anabolic growth PMID: 17030509
17. These results suggested that astaxanthin promoted lipid metabolism rather than glucose utilization during exercise via CPT I activation, which led to improvement of endurance and efficient reduction of adipose tissue with training. PMID: 18082622
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亚细胞定位：

Mitochondrion outer membrane; Multi-pass membrane protein.
蛋白家族：

Carnitine/choline acetyltransferase family
数据库链接：

KEGG: mmu:12894

STRING: 10090.ENSMUSP00000025835

UniGene: Mm.18522

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.