Mouse Cardiotrophin-like cytokine factor 1(CLCF1) ELISA kit

中文名称：

小鼠心脏营养素样细胞因子1(CLCF1)酶联免疫试剂盒
货号：

CSB-EL005479MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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小鼠心脏营养素样细胞因子1(CLCF1)酶联免疫试剂盒（CSB-EL005479MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CLCF1含量。CLCF1 即睫状神经营养因子样细胞因子 1，可参与多种生理病理过程。在研究机制方面，它能与细胞表面受体结合，激活下游信号通路，影响细胞生长、分化、存活等。对其深入研究有望为相关疾病如神经系统疾病、癌症等治疗提供新靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品可广泛应用于基础科研领域，如心血管疾病动物模型中CLCF1的分泌动态研究、体外培养细胞因子释放机制分析，以及心脏组织损伤修复相关的分子机制探索。实验操作流程标准化，兼容常规酶标仪检测，为研究者提供稳定高效的CLCF1定量分析工具，满足神经科学、心血管研究及免疫调节等方向的实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Clcf1 ELISA kit; Bsf3 ELISA kit; Nnt1Cardiotrophin-like cytokine factor 1 ELISA kit; B-cell-stimulating factor 3 ELISA kit; BSF-3 ELISA kit; Novel neurotrophin-1 ELISA kit; NNT-1 ELISA kit
缩写：

CLCF1
Uniprot No.：

Q9QZM3
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

23.5 pg/mL-1500 pg/mL
灵敏度：

5.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CLCF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	89
	Range %	85-93
1:4	Average %	103
	Range %	98-109
1:8	Average %	85
	Range %	80-90

回收率：

The recovery of mouse CLCF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-100
EDTA plasma (n=4)	91	85-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.401	2.293	2.347	2.204
750	1.927	1.876	1.902	1.759
375	1.315	1.361	1.338	1.195
187.5	0.869	0.837	0.853	0.710
94	0.574	0.584	0.579	0.436
47	0.338	0.319	0.329	0.186
23.5	0.232	0.245	0.239	0.096
0	0.146	0.140	0.143

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development. Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
基因功能参考文献：
1. Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders. PMID: 26198769
2. CLC plays a role in astrocyte differentiation via STAT3 activation within the developing brain PMID: 12090754
3. IL-6 (gp130) family cytokine members include CLC/NNT1/BSF3 and IL-27 [review] PMID: 15253382
4. In this study, Clc knockout mice had decreased facial motility, did not suckle, died within 24 hours, and had 32% and 29% fewer motor neurons in the facial nucleus and lumbar ventral horn, respectively. PMID: 19098279
亚细胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
数据库链接：

KEGG: mmu:56708

STRING: 10090.ENSMUSP00000045562

UniGene: Mm.347919

Mouse Cardiotrophin-like cytokine factor 1(CLCF1) ELISA kit

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