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  4. Mouse C-X-C motif chemokine 14(CXCL14) ELISA kit

Mouse C-X-C motif chemokine 14(CXCL14) ELISA kit

说明书下载
  • 中文名称：
    小鼠C-X-C趋化因子14（CXCL14）酶联免疫试剂盒
  • 货号：
    CSB-EL006244MO
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    本试剂盒（CSB-EL006244MO）采用双抗体夹心法原理，专为定量检测小鼠血清、血浆样本中的C-X-C趋化因子14（CXCL14）设计。CXCL14作为免疫调节因子，广泛参与炎症反应、组织发育和肿瘤微环境调控，其表达水平与代谢性疾病、感染性疾病及肿瘤发生发展密切相关，是免疫学与病理机制研究的重要靶点。试剂盒检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL，可精准捕获低丰度目标分子。实验操作包含预包被特异性抗体的96孔板、生物素标记检测抗体及标准品，通过显色反应实现目标蛋白的定量分析，适用于探索CXCL14在免疫性疾病模型、肿瘤微环境动态变化、代谢调控网络中的表达规律及其分子机制研究。该产品严格限定用于科学研究领域，可为探索CXCL14在细胞信号传导、宿主-病原体互作或疾病动物模型中的功能提供可靠检测工具。
  • 别名：
    Cxcl14 ELISA Kit; Bmac ELISA Kit; Kec ELISA Kit; Ks1 ELISA Kit; Mip2g ELISA Kit; Scyb14C-X-C motif chemokine 14 ELISA Kit; B-cell and monocyte-activating chemokine ELISA Kit; Chemokine BRAK ELISA Kit; Kidney-expressed chemokine CXC ELISA Kit; MIP-2G ELISA Kit; Small-inducible cytokine B14 ELISA Kit
  • 缩写：
    CXCL14
  • Uniprot No.：
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 84
    Range % 80-92
    1:2 Average % 103
    Range % 99-108
    1:4 Average % 97
    Range % 92-101
    1:8 Average % 89
    Range % 84-93
  • 回收率：
    The recovery of mouse CXCL14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 97 92-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 2.737 2.652 2.695 2.547
    10 2.235 2.276 2.256 2.108
    5 1.533 1.604 1.569 1.421
    2.5 0.915 0.938 0.927 0.779
    1.25 0.557 0.524 0.541 0.393
    0.625 0.312 0.327 0.320 0.172
    0.312 0.239 0.229 0.234 0.086
    0 0.146 0.150 0.148 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Cxcl14 antibodies

Cxcl14 Proteins

靶点详情

  • 功能：
    Chemotactic for CESS B-cells and THP-1 monocytes, but not T-cells.
  • 基因功能参考文献：
    1. Platelets are a relevant source of CXCL14. Platelet-derived CXCL14 at the site of vascular lesions might play an important role in vascular repair/regeneration. PMID: 28359053
    2. CXCL14 Tg mice showed a suppressed rate of carcinogenesis, decreased tumour volume, and reduced pulmonary metastasis, as well as an increased survival rate of mice following tumour cell injection. PMID: 25765541
    3. CXCL14 does not seem to play a pivotal role during influenza and Escherichia coli infections of the lung. PMID: 25313607
    4. CXCL14 was able to promote bone metastasis through enhancement of cancer cell tropism to the bone and/or recruitment of bone marrow cells around metastatic cancer cells. PMID: 24534874
    5. In conclusion, our results suggested the important function of Cxcl14 in uNK cells and the proper level of Cxcl14 protein were required to recruit NK cells to pregnant uterus. PMID: 23688424
    6. the transient expression of CXCL14 by Purkinje cells in the developing cerebellum, suggesting that it must be involved in the postnatal maturation of the cerebellum PMID: 22843118
    7. CXCL14 may play an important role in central nervous system regulation of feeding behavior PMID: 20428232
    8. Study identifies CXCL14 as a novel marker of tendon connective tissue. PMID: 21038449
    9. Murine CXCL14 is dispensable for the homeostatic recruitment of antigen-presenting cells toward the periphery and for LC functionality. PMID: 17130243
    10. CXCL14 is a critical chemoattractant of white adipose tissue macrophages and a novel regulator of glucose metabolism that functions mainly in skeletal muscle. PMID: 17724031
    11. These results suggest that CXCL14 plays a causal role in high-fat diet-induced obesity. PMID: 17971304
    12. Data suggest that despite the structural homology and similarity in tissue distribution of human and murine CXCL14, distinct differences point to diverse, species-specific needs for CXCL14 in epithelial immunity. PMID: 18809336
    13. findings demonstrate that early overexpression of PMP22 in a mouse model of Charcot-Marie-Tooth disease type 1A results in a strong up-regulation of CXCL14 which seems to play a novel regulatory role in Schwann cell differentiation PMID: 19111616
    14. CXCL14 is an important paracrine/autocrine modulator regulating trophoblast outgrowth at the maternal-fetal interface during the process of pregnancy establishment. PMID: 19626669
    15. These data indicate the possibility that BRAK expression inhibits tumor cell establishment by regulating interactions between tumor stem cells and NK cells and/or suppressing formation of tumor microvessels. PMID: 19887729

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 组织特异性：
    Highly expressed in brain, lung, ovary, muscle and in kidney and liver parenchyma, and at lower levels in bone marrow.
  • 数据库链接：

    KEGG: mmu:57266

    STRING: 10090.ENSMUSP00000021970

    UniGene: Mm.30211

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |