Mouse C-C motif chemokine 28(CCL28) ELISA kit

Mouse C-C motif chemokine 28(CCL28) ELISA kit

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中文名称：

小鼠C-C趋化因子28（CCL28）酶联免疫试剂盒
货号：

CSB-EL004792MO
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

小鼠C-C趋化因子28（CCL28）酶联免疫试剂盒（CSB-EL004792MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL28含量。CCL28，又称粘膜相关上皮趋化因子（MEC），是一种CC趋化因子，存在于几乎所有粘膜组织中，具有免疫刺激作用。CCL28能与CCR3和CCR10结合，介导免疫反应、病毒感染、癌症及抗菌作用。研究显示，CCL28-CCR10通路在类风湿关节炎中促进单核细胞迁移，可能参与滑膜炎症和骨破坏。试剂盒检测范围为78 pg/ml- 5000 pg/ml，灵敏度为19.5 pg/ml。可广泛用于探究感染性疾病、自身免疫反应或黏膜屏障功能异常的小鼠模型中CCL28的动态变化，为黏膜免疫调控机制、炎症通路分析及药物干预效果评估提供可靠工具；尤其在肠道和呼吸道相关疾病机制研究中具有重要价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Ccl28 ELISA Kit; Scya28 ELISA Kit; C-C motif chemokine 28 ELISA Kit; Small-inducible cytokine A28 ELISA Kit
缩写：

CCL28
Uniprot No.：

Q9JIL2
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 pg/ml - 5000 pg/ml
灵敏度：

19.5 pg/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CCL28 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-105
1:2	Average %	92
	Range %	88-95
1:4	Average %	86
	Range %	80-94
1:8	Average %	89
	Range %	84-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

5000

2.692

2.921

2.807

2.681

2500

2.157

2.166

2.162

2.036

1250

1.469

1.522

1.496

1.370

625

0.866

0.848

0.857

0.731

312

0.523

0.545

0.534

0.408

156

0.324

0.336

0.330

0.204

0.233

0.252

0.243

0.117

0.129

0.122

0.126

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Chemotactic for resting CD4, CD8 T-cells and eosinophils. Binds to CCR10 and induces calcium mobilization in a dose-dependent manner.
基因功能参考文献：
1. Blockade of CCL28 or CCR10 during an acute severe respiratory paramyxoviral infection is sufficient to prevent the development of post-viral airway disease. However, the mechanism of action is unclear and requires further exploration. PMID: 28474501
2. These data suggest that CCL28 could be useful in enhancing the IgA immune response that will likely play a pivotal role in prophylactic HIV vaccines. PMID: 22066023
3. tumour hypoxia promotes the recruitment of regulatory T (T(reg)) cells through induction of expression of the chemokine CC-chemokine ligand 28 (CCL28), which, in turn, promotes tumour tolerance and angiogenesis PMID: 21753853
4. demonstrate that interactions between the antimicrobial C-terminus of CCL28 with an appropriate CC chemokine N-terminal domain is important for the full antimicrobial activity of CCL28. PMID: 19830739
5. CCL28 has dual functions in mucosal immunity as a chemokine attracting cells expressing CCR10 and/or CCR3 and also as an apically secreted molecule with a potent antimicrobial activity against a broad spectrum of microbes. PMID: 12538707
6. MEC attracts IgA- but not IgG- or IgM-producing plasmablasts from both intestinal and nonintestinal lymphoid and effector tissues, including the intestines, lungs, and lymph nodes draining the bronchopulmonary tree and oral cavity. PMID: 12646646
7. Epithelial cells and venular endothelium of both small intestine and colon are positive for CCL28; CCL28 plays a direct role in intestinal homing of IgA antibody-secreting cells by mediating their extravasation into intestinal lamina propria. PMID: 15356112
8. CCL28 is a key regulator of IgA ASC accumulation in the mammary gland and thus controls the passive transfer of IgA antibodies from mother to infant. PMID: 15381732
9. CCL28 appears to play a role in regulating eosinophil recruitment to peribronchial regions of the lung possibly by coordinated temporal production with eotaxin PMID: 15681819
10. CCL28 mediates mucosal immunity in HIV exposure and infection PMID: 17912348
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亚细胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
组织特异性：

Mainly expressed in testis, epithelial cells of normal colon, kidney, Peyer patches, lymph nodes. Also found in lower levels in brain, spleen and lung.
数据库链接：

KEGG: mmu:56838

STRING: 10090.ENSMUSP00000096847

UniGene: Mm.143745

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.