Mouse Bone marrow proteoglycan(PRG2) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠骨髓蛋白多糖(PRG2)酶联免疫试剂盒（CSB-EL018670MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PRG2含量。PRG2即前颗粒蛋白 2，是一类具有潜在药用价值的靶点。其与过敏、炎症反应等密切相关，研究机制主要是通过对免疫细胞的调节，在免疫信号传导通路中发挥作用，目前对其深入研究或可为相关疾病治疗带来新策略。试剂盒检测范围为3.9 ng/mL-250 ng/mL，广泛应用于骨髓发育机制研究、炎症性疾病模型评估或血液相关病理变化的分子机制探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Prg2 ELISA Kit; Mbp-1 ELISA Kit; Bone marrow proteoglycan ELISA Kit; BMPG ELISA Kit; Proteoglycan 2) [Cleaved into: Eosinophil granule major basic protein ELISA Kit; EMBP ELISA Kit; MBP)] ELISA Kit
缩写：

PRG2
Uniprot No.：

Q61878
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.9 ng/mL-250 ng/mL
灵敏度：

0.98 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PRG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	89-97
1:10	Average %	103
	Range %	98-107
1:20	Average %	90
	Range %	84-94
1:40	Average %	98
	Range %	92-102

回收率：

The recovery of mouse PRG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	94	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytotoxin and helminthotoxin. MBP also induces non-cytolytic histamine release from basophils. It is involved in antiparasitic defense mechanisms and immune hypersensitivity reactions.
基因功能参考文献：
1. data show that bioactive phospholipids and PRG-2 are critical for guiding thalamic axons to their proper cortical targets. PMID: 27641493
2. The absence of MBP-1 and EPX promoted a concomitant loss of eosinophil lineage-committed progenitors in the marrow, identifying a specific blockade in eosinophilopoiesis as the causative event. PMID: 23736699
3. major basic protein decreases epithelial barrier function and in this manner contributes to the pathogenesis of inflammatory bowel diseases. PMID: 16227527
4. Host protection against Brugia pahangi infections was unimpaired in mice deficient in major basic protein 1. PMID: 16299347
5. Mice deficient for either eosinophil peroxidase or major basic protein on the 129/SvJ background developed significantly higher worm burdens than wild-type mice.(major basic protein 1) PMID: 16926417
6. Eosinophil-derived MBP-1 plays a significant role in regulating muscular dystrophy by attenuating the cellular immune response and promoting tissue fibrosis that can eventually contribute to increased mortality. PMID: 18430716
显示更多

收起更多
亚细胞定位：

Cytoplasmic granule.
数据库链接：

KEGG: mmu:19074

STRING: 10090.ENSMUSP00000028467

UniGene: Mm.142727