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Mouse Aspartate Aminotransferase (AST) ELISA kit

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CUSABIO小鼠天门冬氨酸氨基转移酶（AST）定量检测试剂盒（货号：CSB-E12649m），采用双抗体夹心ELISA技术原理，可精准检测血清、血浆及细胞培养上清样本中的AST含量。AST是氨基酸代谢过程中的关键酶，主要分布于肝脏和心肌细胞，当组织损伤时释放入循环系统，其水平变化常用于科研中评估肝细胞损伤程度及心脏功能状态。该试剂盒具备1.56-100 mIU/mL的宽检测范围，灵敏度达1.56 mIU/mL，可稳定识别小鼠AST蛋白的微小浓度变化，操作流程标准且重复性良好。适用于实验室中肝毒性药物筛选、心脏疾病模型建立、细胞损伤机制研究等场景，例如通过检测细胞培养上清中的AST释放量评价化合物对肝实质细胞的毒性作用，或在小鼠代谢性疾病模型中动态监测血浆AST水平变化。试剂盒提供预包被微孔板及标准化试剂，实验全程无需复杂设备，3.5小时内即可完成检测，为科研人员提供高效、便捷的实验解决方案。
别名：

Got2 ELISA kit; Got-2Aspartate aminotransferase ELISA kit; mitochondrial ELISA kit; mAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.7 ELISA kit; Fatty acid-binding protein ELISA kit; FABP-1 ELISA kit; Glutamate oxaloacetate transaminase 2 ELISA kit; Kynurenine aminotransferase 4 ELISA kit; Kynurenine aminotransferase IV ELISA kit; Kynurenine--oxoglutarate transaminase 4 ELISA kit; Kynurenine--oxoglutarate transaminase IV ELISA kit; Plasma membrane-associated fatty acid-binding protein ELISA kit; FABPpm ELISA kit; Transaminase A ELISA kit
缩写：

GOT2
Uniprot No.：

P05202
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates
检测范围：

1.56 mIU/mL-100 mIU/mL
灵敏度：

0.39 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	93
	Range %	87-98
1:4	Average %	91
	Range %	89-94
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of mouse AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-99
EDTA plasma (n=4)	98	94-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
100	2.915	2.898	2.907	2.720
50	2.475	2.432	2.454	2.267
25	2.015	2.002	2.009	1.822
12.5	1.376	1.342	1.359	1.172
6.25	0.731	0.717	0.724	0.537
3.12	0.472	0.445	0.459	0.272
1.56	0.312	0.301	0.307	0.120
0	0.189	0.185	0.187

本试剂盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse AST antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled AST antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
基因功能参考文献：
1. GOT2-mediated ATP synthesis may be a pivotal mechanism underlying the protective effect of febuxostat against neurodegeneration in EA PMID: 29107957
2. it is quite likely that the down regulation of GOT2 after blast exposure is playing a significant role in mitochondrial dysfunction after blast exposure PMID: 23600763
3. The role of mAspAT in vivo is to control the levels of endogenous kynurenic acid (KYNA) through modulation of the mAspAt in the mouse brain. PMID: 20977429
4. KAT IV inhibited by equimolar tryptophan and aspartate, and half-life is 6.5 minutes at 65 degree C. PMID: 20482848
亚细胞定位：

Mitochondrion matrix. Cell membrane.
蛋白家族：

Class-I pyridoxal-phosphate-dependent aminotransferase family
组织特异性：

Detected in brain (at protein level).
数据库链接：

KEGG: mmu:14719

STRING: 10090.ENSMUSP00000034097

UniGene: Mm.230169

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.