Mouse Apolipoprotein C-II(APOC2) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠载脂蛋白C-II（APOC2）酶联免疫检测试剂盒（货号CSB-EL001932MO），采用竞争法原理实现定量检测，适用于血清、血浆及组织匀浆样本中APOC2的精确分析。APOC2作为脂蛋白代谢的关键调节因子，通过激活脂蛋白脂肪酶参与甘油三酯水解过程，其表达水平与脂代谢疾病、动脉粥样硬化等病理机制密切相关。试剂盒检测灵敏度高，线性范围为18.8-1200 ng/mL，可覆盖生理及病理状态下样本的检测需求，实验操作兼容常规实验室条件，支持96孔板高通量检测。科研人员可通过该试剂盒研究脂代谢异常相关疾病（如肥胖、糖尿病）的分子机制，或评估药物干预对脂代谢途径的影响。试剂盒提供优化的预包被抗体和标准品，样本仅需简单预处理即可上样检测，搭配显色底物系统实现快速读值，实验结果稳定且重复性良好，为心血管疾病及代谢综合征的基础研究提供可靠工具。
别名：

Apoc2Apolipoprotein C-II ELISA kit; Apo-CII ELISA kit; ApoC-II ELISA kit; Apolipoprotein C2 ELISA kit
缩写：

APOC2
Uniprot No.：

Q05020
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

18.8 ng/mL-1200 ng/mL
灵敏度：

9.4 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	97
	Range %	88-102
1:40	Average %	93
	Range %	85-98
1:80	Average %	103
	Range %	96-107
1:160	Average %	104
	Range %	95-109

回收率：

The recovery of mouse APOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	101	94-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.
亚细胞定位：

Secreted.
蛋白家族：

Apolipoprotein C2 family
组织特异性：

Adult and fetal liver, intestine and peritoneal macrophages.
数据库链接：

KEGG: mmu:105886299

STRING: 10090.ENSMUSP00000003074

UniGene: Mm.28394