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Mouse Anti-Cyclic Citrullinated Peptide Antibody (Anti-CCP-antibody) ELISA kit

  • 中文名称:
    小鼠抗环胍氨酸肽抗体(Anti-CCP-antibody)酶联免疫试剂盒
  • 货号:
    CSB-EQ027743MO
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO小鼠抗环胍氨酸肽抗体(Anti-CCP-antibody)定量检测试剂盒(货号:CSB-EQ027743MO),专为科研领域设计,适用于小鼠血清或血浆样本中Anti-CCP抗体的精准分析。Anti-CCP抗体是类风湿性关节炎(RA)等自身免疫疾病研究中的重要生物标志物,其检测可为小鼠疾病模型中的免疫反应机制及药物干预效果评估提供关键数据。试剂盒采用双抗体夹心法原理,通过高特异性抗体对实现靶标捕获与信号放大,检测范围覆盖6.25 U/mL至400 U/mL,可灵敏检测低浓度样本,并满足不同实验需求的高浓度样本分析。试剂盒操作流程标准化,兼容血清和血浆样本前处理,适用于基础科研中疾病机制探索、药物研发阶段的药效学评价以及动物模型中的生物标志物动态监测等场景。实验数据稳定可靠,严格遵循科研级质量控制标准,为自身免疫性疾病相关研究提供高效工具。
  • 缩写:
    Anti-CCP-Ab
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma
  • 检测范围:
    6.25 U/mL-400 U/mL
  • 灵敏度:
    1.56 U/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-CCP-antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 88-101  
    1:2 Average % 97  
    Range % 91-106  
    1:4 Average % 95  
    Range % 90-106  
    1:8 Average % 96  
    Range % 89-104  
  • 回收率:
    The recovery of mouse anti-CCP-antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 90-110  
    EDTA plasma (n=4) 95 83-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    U/ml OD1 OD2 Average Corrected  
    400 2.697 2.741 2.719 2.573  
    200 2.201 2.224 2.213 2.067  
    100 1.507 1.483 1.495 1.349  
    50 0.834 0.849 0.842 0.696  
    25 0.492 0.511 0.502 0.356  
    12.5 0.297 0.310 0.304 0.158  
    6.25 0.226 0.238 0.232 0.086  
    0 0.148 0.144 0.146    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

平均分:
5.0分 - 2 个评价

样品类型:Serum

样品信息:小鼠

稀释比:其他

产品评价: 我用anti-CCP试剂盒检测小鼠正常、模型、给药组的血清样本,数据比较稳定,组内差异小。个别样本复测值差别也很小,试剂盒质量稳定。以前买的试剂盒剩余了几个孔,大概过了半年多再用,还是很不错,赞!希望华美继续保持!

By 吕老师

样品类型:Serum

样品信息:小鼠

稀释比:其他

产品评价: 试剂盒质量很好,测试很稳定,标曲也很好。

By 宾老师