Mouse Angiopoietin-related protein 7(ANGPTL7) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠血管生成素相关蛋白7（ANGPTL7）酶联免疫检测试剂盒（货号：CSB-EL001715MO），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的ANGPTL7蛋白，检测范围为6.25 pg/mL至400 pg/mL，灵敏度可达6.25 pg/mL。ANGPTL7是血管生成素样蛋白家族成员，参与血管生成调控和细胞代谢过程，其表达异常与眼部疾病、纤维化等病理机制相关。本试剂盒通过特异性抗体对靶蛋白进行高灵敏度捕获与检测，适用于小鼠模型中ANGPTL7蛋白水平的定量分析，可应用于心血管疾病、代谢调控或组织纤维化等领域的机制研究，例如在基因编辑模型或药物干预实验中评估ANGPTL7的动态变化。实验操作兼容常规酶标仪分析，提供标准品及预包被酶标板，需自备部分试剂。本产品仅限科学研究使用。
别名：

Angptl7Angiopoietin-related protein 7 ELISA kit; Angiopoietin-like protein 7 ELISA kit
缩写：

ANGPTL7
Uniprot No.：

Q8R1Q3
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANGPTL7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-94
1:2	Average %	98
	Range %	93-103
1:4	Average %	102
	Range %	95-109
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of mouse ANGPTL7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-101
EDTA plasma (n=4)	93	88-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Has a role in the formation and organization of the extracellular matrix. In the eye, it functions as a mediator of dexamethasone-induced matrix deposition in the trabecular meshwork, the tissue responsible for the outflow of the ocular aqueous humor and for the maintenance of intraocular pressure. Is a negative regulator of angiogenesis in the cornea, and plays a major role in maintaining corneal avascularity and transparency.
基因功能参考文献：
1. Angptl7 is a factor that promotes pro-inflammatory responses in macrophages through the P38 MAPK signaling pathway PMID: 26973239
2. Angptl7 was indispensable for bone marrow microenvironment to support hematopoietic stem cells repopulation PMID: 25652910
亚细胞定位：

Secreted.
数据库链接：

KEGG: mmu:654812

STRING: 10090.ENSMUSP00000030840

UniGene: Mm.388929